high performance liquid chromatography (hplc) with diode array detection (dad) and electrospray ionization tandem mass spectrometry (esi/ms/ms) was used to analyze the main components in the methanol extract of fructus aurantii (fa) and the metabolites in rat biological samples after oral administration of the methanol extract of fa. there were 31 constituents identified in the extract of fa in...

A method for the analysis of flavonoids in Astragali Radix by high-performance liquid chromatography (HPLC) combined with photodiode-array detection (DAD) and an electrospray ionization (ESI)--mass spectrometry (MS) was developed. After the samples were extracted with ethanol, the optimum separation conditions for these analytes were achieved using a gradient elution system and a 2.0 x 150 mm S...

The diversifi ed genus Passifl ora is well distributed all over Brazil, and many species have been long used as medicinal plants, mainly against anxiety disturbances. This effect has been attributed to its rich fl avonoid composition. Flavonoids’ main class, fl avonoid glycosides, has presented central action, particularly as sedativehypnotic, anxiolytic and analgesic. The objective of the pres...

In the present study a universally applicable HPLC-DAD/ESI-MS/MS method was developed for carrying out the comprehensive characterization of Jitai tablets (JTT). Based on the ESI-MS(n) fragmentation patterns of the reference standards, a total of 101 components were identified or tentatively characterized by comparing their retention times, UV and MS spectra with those of reference standards or...

Phenolic compounds are abundant secondary metabolites in plums, with potential health benefits believed to be due to their antioxidant activity, amongst others. Phenolic characterisation of South African Prunus salicina Lindl. plums is necessary to fully evaluate their potential health benefits. An HPLC method using diode-array detection (DAD) for quantification of phenolic compounds was improv...

Metabolite identification is a key step for metabonomics study. A fully automated hyphenation of HPLC-diode-array detector (DAD) mass spectrometry (MS) solid phase extraction (SPE)-NMR spectroscopy (HPLC-DAD-MS-SPE-NMR) is one of the most efficient methods to determine the structure of a given unknown metabolite in a complex mixture (metabonome) and hence represents one of the most important an...

Terminalia albida (Combretaceae), widely used in Guinean traditional medicine, showed promising activity against Plasmodium falciparum and Candida albicans previous studies. Bioassay-guided fractionation was carried out order to isolate the compounds responsible for these activities. Fractionation isolation were performed by flash chromatography, followed semi-preparative HPLC-DAD-MS. The struc...