p-nonylphenol (p-np) as a non ionic surfactant is used in many industries such as pesticides, cosmetics,detergents and so on. our previous study showed that the p-np causes reduction in viability of the rats bonemarrow mesenchymal stem cells (mscs). the aim of the present study was to investigate the mechanism of the cell death due to p-np exposure. the cells, after 3rd passage were treated wit...

in this study organotypic adult spinal cord slices were used to investigate whether caspases could participate in the apoptosis of motor neurons. the thoracic region of spinal cord was sliced using a tissue chopper and cultured in a medium for 6h. morphological and biochemical features of apoptosis were assessed by fluorescent staining and terminal deoxynucleotidyl nick end labeling (tunel) met...

Apoptosis plays a central role in the complex balance between invading pathogen and host defense. Depending on the pathogen, apoptosis of host cells may be beneficial or detrimental to survival. Studies using a clinically relevant intra-abdominal model of sepsis (peritonitis) have indicated that two types of cells, lymphocytes and gastrointestinal epithelial cells, undergo accelerated apoptosis...

PURPOSE To investigate whether apoptosis plays a notable role in degeneration of corneal endothelial cells in patients with Fuchs' dystrophy. METHODS Forty-seven corneal buttons from 41 patients with Fuchs' dystrophy were studied. Nucleus labeling, transmission electron microscopy (TEM), and TdT-dUTP terminal nick-end labeling (TUNEL) were used to detect apoptosis. TEM and TUNEL were performe...

This study determined whether retinal degeneration during diabetes includes retinal neural cell apoptosis. Image analysis of retinal sections from streptozotocin (STZ) diabetic rats after 7.5 months of STZ diabetes identified 22% and 14% reductions in the thickness of the inner plexiform and inner nuclear layers, respectively (P < 0. 001). The number of surviving ganglion cells was also reduced...

background: pectin is composed of complex polysaccharides that can inhibit cancer metastasis and proliferation with no evidence of toxicity. in the present study, the apoptotic and necrotic effects of pectic acid (pa) on the rat pituitary gh3/b6 tumor cells has been investigated. methods: gh3/b6 cells were cultured in the ham’s f12 medium enriched with 15% horse serum and 2.5% fetal bovine seru...

Several studies have reported the occurrence and significance of programmed cell death (apoptosis) of testicular germ cells in mammals. In those studies, apoptotic germ cells were identified by morphological criteria or by in situ end labeling (TUNEL) and were enumerated from histological sections by semi-quantitative and time-consuming techniques. In the present study, we have established a fl...

Background Oleanolic acid (OA) has notable anti-inflammatory and anti-tumor effects, but the role of OA in cerebral ischemia-reperfusion injury (CIRI) not been reported so far. Methods Oxygen glucose deprivation/reoxygenation (OGD/R) model was induced PC12 cells. MTT assay used to detect cell viability cells, while ELISA detected expression TNF-?, IL-1? IL-6. The superoxide dismutase (SOD), mal...

BACKGROUND Resveratrol, a natural isolate from plant sources, has a long and important history in traditional Chinese medicine. In the present study we investigated the effect of resveratrol on human colon cancer cell lines. MATERIAL/METHODS We used the Cell Counting kit-8 (CCK-8) for determination of colon cancer cell viability. Apoptosis induction was analyzed using the DeadEnd™ Colorimetri...