Continuous exposure of cells to exogenous and endogenous agents produces many types of DNA damage during normal cell cycles. Post-replication repair, consisting of error-free and error-prone sub-pathways, is required for tolerance of such DNA damage. REV1 plays a crucial role in regulation of the error-prone pathway. To facilitate analysis of its cellular functions, we here generated a mouse Re...

Lipid hydroperoxide may react with protein or amino phospholipid without secondary decomposition. We prepared a polyclonal an tibody to lipid hydroperoxide-modified proteins using 13S-hydroperoxy-9Z, 1 1E-octadecadienoic acid-modified keyhole limpet hemocyanin (13-HPODEKLH) as immunogen. The antibody recognized 13-HPODEmodified bovine serum albumin (BSA), but not aldehydemodified proteins, such...

paraneoplastic syndromes result from secretion of hormones, peptides or cytokines by tumor or immune cross-reactivity between malignant and normal tissues. these conditions are rare in children, but when the clinical presentation of patients with a tumor is unusual, these syndromes should be emphasized . extrarenal tumors with renin-secretion are rare in children. they may be related to paraneo...

MicroRNAs are widely studied as circulating biomarkers for early stage diagnosis of several diseases. Detection and quantification miRNAs is currently performed through complex time consuming procedures. Herein we demonstrate a rapid, multiplex, one-pot detection method based on two-step amplification the signal measured by Reflective Phantom Interface (RPI) label-free optical biosensor. We ach...

Norovirus (NoV) capsid proteins were expressed as virus-like particles (VLPs) by using recombinant baculovirus in insect cells, which had 5 genotypes in genogroup I and 11 genotypes in genogroup II, and the VLPs were used as immunogens. Polyclonal antibody against the VLP of GII/3 genotype showed broad-range cross-reactivity, reacting not only with intra-genogroup strains, but also inter-genogr...

The analysis and polyclonal antibody response for newly synthesized maltose-BSA conjugate neoglycoproteins is described. In this first proof of concept study, a simple carbohydrate antigen, maltose, was linked to BSA by reductive amination. An aglycone spacer was utilized to conserve the intact annular maltose structure and to promote the accessibility of the carbohydrate immunogen hapten durin...

nowadays immunochemical techniques have played a very important and valuable role in quantitative and qualitative assays of liquid compounds of the body. producing antibody against immunogenes is the first step to make immunochemical kits. in this study production and purification of polyclonal antibody against melatonin has been considered. this hormone which has several important functions in...

Purpose: Among all known human coronaviruses, some viruses (e.g., SARS-CoV-2) cause severe pneumonia or even death. With the regard to its spread and importance of rapid identification/treatment, because pAbs are relatively cheap, able bind more sites on antigens neutralize them, this study was done for production purification anti-SARS-CoV-2 polyclonal antibodies (pAb) in rats. Methods: Viral ...

Antibody therapies to prevent or limit filovirus infections have received modest interest in recent years, in part because of early negative experimental evidence. We have overcome the limitations of this approach, leveraging the use of antibody from nonhuman primates (NHPs) that survived challenge to filoviruses under controlled conditions. By using concentrated, polyclonal IgG antibody from t...