Mammalian spermatozoa are characterized by a high proportion of polyunsaturated fatty acids (PUFA) which play a crucial role in fertilization. This review focuses on analysis of sperm fatty acid profiles and the effects of omega-3, saturated and trans dietary and sperm fatty acids on sperm parameters. Two major points have been pivotal points of investigation in the field of sperm fatty acid pr...

The epitope structure of the human sperm antigen reacting with antibodies present in sera of infertile women has been studied using mAb H6-3C4, which produces immobilization of human sperm in the presence of complement. Another antibody, NUH2, which also induces human sperm immobilization, was used to substantiate the presence of a receptor on sperm involved in susceptibility to immobilization....

One of the most common lesions present in the spermatozoa of human infertility patients is an idiopathic failure of sperm-egg recognition. Although this unique cellular interaction can now be readily by-passed by assisted reproductive strategies such as intracytoplasmic sperm injection (ICSI), recent large-scale epidemiological studies have encouraged the cautious use of this technology and hig...

BACKGROUND Human sperm DNA damage may adversely affect reproductive outcomes, and the spermatozoa of infertile men possess substantially more DNA damage than that of fertile men. To date, there is no available treatment for men with high levels of sperm DNA damage. The objective of this study was to examine the effect of varicocelectomy on sperm DNA denaturation (DD, an index of sperm DNA damag...

PURPOSE Sperm immobilization factor isolated from Staphylococcus aureus immobilizes human spermatozoa as well as motile bacteria, showing that sperm immobilization factor receptor might be shared by bacteria and human spermatozoa. Thus, we sought to identify a common sperm immobilization factor binding receptor on spermatozoa and bacteria. MATERIALS AND METHODS Sperm immobilization factor was...

INTRODUCTION It is widely recognized that all that fathers essentially contribute to the next generation is half of their genome, and mature sperm cells are transcriptionally and translationally inactive. However, an increasing number of reports have shown that RNAs were present in human sperm detected by using RT-PCR, differential display, in situ hybridization and microarrays [1]. One study s...

OBJECTIVE To evaluate the effects of laptop computers connected to local area networks wirelessly (Wi-Fi) on human spermatozoa. DESIGN Prospective in vitro study. SETTING Center for reproductive medicine. PATIENT(S) Semen samples from 29 healthy donors. INTERVENTION(S) Motile sperm were selected by swim up. Each sperm suspension was divided into two aliquots. One sperm aliquot (experime...

Trichomonas vaginalis infection is one of the most prevalent sexually transmitted infections in humans and is now recognized as an important cause of infertility in men. There is little information about the effect of extracellular polymeric substances (EPS) from T. vaginalis on sperm, but previous reports do not provide a conclusive description of the functional integrity of the sperm. To inve...

The active interest in determining the ultraThe washed sperm samples were then treated with structural organization of ejaculated human sperm alkaline thioglycolate (18) . A 0 .8 M concentration of nucleus by the normal thin-sectioning technique alkaline thioglycolate was prepared by raising the has led to a state of understanding that is still pH to 9 with I N NaOH. This reagent was mixed inco...