Τhis study focuses on the exploitation of food-industry wastes as a source for hydrogen production. The wastes which were used were out of date solid baby foods at seven different flavors (solid wastes) and a glucose-based syrup (liquid waste). Preliminary batch hydrogen experiments at mesophilic conditions and initial carbohydrates concentration of 5 g/L were performed to assess the hydrogen p...

−Five pretreatment methods, namely chemical, acid, heat-shock, freezing and thawing, and base, were evaluated for the enrichment of hydrogen-producing bacteria in anaerobic granulated sludge, which will be subsequently used as seed in biological hydrogen production. All the pretreatments showed positive effects towards improving hydrogen (H 2 ) generation by the microbial population with higher...

As a clean energy source and industrial material, hydrogen is very valuable. Electrolysis of water and chemical methods are well-known for producing hydrogen, however, all of these methods need additional energy supply. Besides highly energy cost, the chemical methods will lead to serious environment pollution. Compared with traditional methods, biological production of hydrogen has showed sign...

BACKGROUND Molecular hydrogen is an environmentally-clean fuel and the reversible (bi-directional) hydrogenase of the cyanobacterium Synechocystis sp. PCC 6803 as well as the native Escherichia coli hydrogenase 3 hold great promise for hydrogen generation. These enzymes perform the simple reaction 2H+ + 2e- <--> H2 (g). RESULTS Hydrogen yields were enhanced up to 41-fold by cloning the bidire...

The production of deuterated methane by Methanobacterium thermoautotrophicum in H2O-D2O mixtures was examined by high-resolution mass spectrometry. The hydrogen in the methane arose solely from water and not from hydrogen gas. Hydrogen gas served only as an electron source in methanogenesis. A whole-cell product isotope discrimination of 1.5 favoring hydrogen over deuterium was observed in meth...