The effect of gelatinization of dietary starch (wheat, corn and potato) on the distribution of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase along the small intestine of rats was studied in villus and crypt cell populations isolated by graduated scraping. Gelatinized starch preferentially stimulated jejunal reductase activity in both villus and crypt cells and consequently retained ...

NADH and NAD+ are neither substrates nor inhibitors of 3-hydroxy3-methylglutaryl coenzyme A (HMGCoA) reductase in concentrations up to 1 mM. Addition of either NADH or NAD+ enhanced the activity of rat liver microsomal reductase, yet NADH failed to affect the activity of the freeze-thaw solubilized enzyme. The degree of enhancement of enzyme activity by NADH decreased as GSH concentration in th...

BACKGROUND Experimental animal data suggest that 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors (statins) might reduce enhanced sympathetic activity, a hallmark of hypertensive patients. This hypothesis was tested for the first time in patients with primary hypertension. METHODS AND RESULTS Using a prospective, randomized, placebo-controlled, double-blind, cross-over des...

OBJECTIVE Inhibition of 3hydroxy3methylglutaryl-coenzyme A (HMG-CoA) reductase by statins and the subsequent reduction in Rho protein isoprenylation inactivates these important signaling molecules. The purpose of this study was to directly monitor statin effects on Rho proteins. METHODS AND RESULTS We used biphasic Triton X-114 system, 1-dimensional isoelectric focusing, and 2D-electrophoresi...

3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) catalyzes the conversion of HMG-CoA to mevalonate using NADPH and the enzyme is involved in rate-controlling step of mevalonate. Inhibition of HMGR is considered as effective way to lower cholesterol levels so it is drug target to treat hypercholesterolemia, major risk factor of cardiovascular disease. To discover novel HMGR inhibitor, we p...

The effects of atorvastatin on the expression of the hepatic HMG-CoA reductase and LDL receptor genes were investigated in rats. Like the other statins, atorvastatin increased the rate of degradation and presumably cycling of the hepatic LDL receptor. In atorvastatin-treated rats, the half-life of the receptor was decreased by over 60%. Hepatic HMG-CoA reductase mRNA levels were increased about...

OBJECTIVES We sought to evaluate the effects of hydroxymethylglutaryl coenzyme A (HMG-CoA) reductase inhibitors on vascular smooth muscle cell (VSMC) proliferation in vitro and neointimal formation in vivo after vascular injury. BACKGROUND Neointimal hyperplasia after vascular injury is responsible for restenosis after arterial stenting, whereas arterial remodeling and neointimal formation ar...

1. The activities of acetoacetyl-CoA thiolase, hydroxymethylglutaryl-CoA synthase and lyase and acetoacetyl-CoA deacylase were measured in homogenates of samples of liver, rumen epithelium (long papillae), kidney and lactating mammary gland derived from slaughtered cows. 2. The activities of the four enzymes in bovine liver were similar to the activities previously reported for the correspondin...

BACKGROUND Studies in dogs showed that some hydroxymethylglutaryl coenzyme A reductase inhibitors (statins) are associated with cataract when administered in excessive doses. Clinical safety data of statins regarding cataract development in humans have been of limited value so far. OBJECTIVE To determine whether long-term use of statins is associated with an increased risk of cataract. METH...

We did a secondary analysis of data from three large colorectal adenoma chemoprevention trials to assess the association between 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor use and reduced risk of recurrent colorectal adenomas. Reported use of HMG-CoA reductase inhibitors was not associated with a reduced recurrence of colorectal adenomas, multiple adenomas, or advanced ...