in the present work a modified in-tube solid phase microextraction technique followed by gas chromatography-mass spectrometry (gc-ms) has been applied for determination of water odorants. geosmin, 2-methyl isoborneol, 2-isopropyl-3-methoxy pyrazine, 2-isobutyl-3-methoxy pyrazine and 2,4,6-trichloroanisol are of the most important odorants which have been studied. in the described method, analyt...

Human monocytes cultured on surface-bound immune complexes exhibited a loss of ability to form rosettes with IgG-sensitized sheep erythrocytes (EA). This loss was not a result of inhibition of Fc receptors by solubilized complexes nor of release of soluble factors by the cells. Loss of EA rosetting was not prevented by culture of monocytes at 4 degrees C, or by treatment with colchicine, cytoch...

In the title compound {systematic name: [2-(3-cyano-4-isobutyl-oxyphen-yl)-4-methyl-1,3-thia-zole-5-carb-oxy-lic acid (febuxostat) methanol monosolvate}, C(16)H(16)N(2)O(3)S·CH(4)O, the benzene and thia-zole rings in the febuxostat mol-ecule are twisted at 5.3 (1)°. In the crystal structure, inter-molecular O-H⋯O and O-H⋯N hydrogen bonds link the febuxostat and methanol mol-ecules into helical ...

Introduction: To evaluate the cAMP -mediated IBMX (3-IsoButyle -1-Methyl Xanthin) and db-cAMP (dibutyryl cAMP) effects on differentiation of human Embryonic Stem Cells (hESCs) into nerve cells were the objectives of this study. Methods: We have used Royan H1 hESC- derived embryoid bodies with four treatment groups: six days treatment with IBMX (5×10 -4M) and db-cAMP (10 -9M) (referred to as...

Abstract Study question Can the transfer of aggregated chromosomes (AC) as compared to spindle-chromosome (SCT) reduce mitochondrial DNA (mtDNA) carryover and improve development rates? Summary answer The rate mtDNA after AC was only 0.1%, which much less than that 2.0% SCT. What is known already Nuclear techniques such SCT pronuclear have been applied prevent diseases treat early embryonic arr...

The antioxidant activity of Annona squamosa (A. squamosa) leaf was carried out using methanol extract and fractionated namely n-hexane, ethyl acetate residue. performed the 1-diphenyl-2-Pycrilhydrazil (DPPH) method. test residue yielded IC50 6.87, 169.99, 31.55 44.75 ppm. fraction with ppm by column chromatography silica gel G60 as stationary phase n-hexane: mobile phase. results obtained 181 f...

BACKGROUND Type III polyketide synthases (PKSs) contribute to the synthesis of many economically important natural products, which are typically produced by direct extraction from plants or synthesized chemically. For example, humulone and lupulone (Fig. 1a) in hops (Humulus lupulus) account for the characteristic bitter taste of beer and display multiple pharmacological effects. 4-Hydroxy-6-me...

Experimental evaluation of a diffusion reaction model (part 1) for the determination of both steady-and transient-state behavior of biofilters for waste air biotreatment is presented. The model, applied to the aerobic biodegradation of methyl ethyl ketone (MEK) and methyl isobutyl ketone (MIBK) vapors from air as single and as mixed pollutants, proved appropriate for describing most of the expe...

Conversion of 3T3-L1 preadipocytes to fully developed adipocytes in culture under the influence of dexamethasone, 1-methyl-3-isobutyl xanthine, and insulin offers a unique system to investigate differentiation-related changes in lipid metabolism. Depending on the type of isotopic precursors ([3H2]O, 32Pi, and [1-14C]acetate) used, a 10-170-fold increase in the rate of incorporation into lipid w...