Prostaglandin E2 (PGE2) is a potent lipid molecule with complex proinflammatory and immunoregulatory properties. PGE2 can shape the immune response by stimulating the production of IgE antibody by B lymphocytes and the synthesis of T-helper type 2 cytokines [e.g., interleukin (IL)-4, IL-10], while inhibiting production of Th1 cytokines (e.g., interferon-gamma, IL-12). It is unknown what type of...

Potential triggers for equine asthma are allergens from hay and straw dusts, mold spores storage mites. The contribution of these environmental trigger factors to is still largely uncertain. aim this study was compare results four allergy tests healthy asthma-affected horses, evaluate the clinical relevance tested positive via specific inhalation provocation test. Fifteen horses were classified...

The purpose of this study was to define the operational and performance characteristics of a commercially available monoclonal antibody based (mac) ELISA for detection of allergen-specific IgE in dogs. The average intra-assay variance over 1 year was 9.7% (range 2.5-62.7%), while the interassay variance averaged 10.8% (range 8.1-13.8%). The average positive control responses observed for grass,...

BACKGROUND Imidacloprid has been commonly used as a pesticide for crop protection and acts as nicotinic acetylcholine receptor agonists. Little information about the relationship between imidacloprid and allergy is available. OBJECTIVE This study aims to examine the effects of imidacoprid on IgE-mediated mast cell activation. METHODS The rat basophilic leukemia cell line RBL-2H3 (RBL-2H3 ce...

Large scale aggregation of fluorescein-labeled immunoglobulin E (IgE) receptor complexes on the surface of RBL cells results in the co-aggregation of a large fraction of the lipophilic fluorescent probe 3,3'-dihexadecylindocarbocyanine (diI) that labels the plasma membranes much more uniformly in the absence of receptor aggregation. Most of the diI molecules that are localized in patches of agg...

Gounni, Abdelilah Soussi. The high-affinity IgE receptor (Fc RI): a critical regulator of airway smooth muscle cells? Am J Physiol Lung Cell Mol Physiol 291: L312–L321, 2006. First published March 31, 2006; doi:10.1152/ajplung.00005.2006.—The airway smooth muscle (ASM) has been typically described as a contractile tissue, responding to neurotransmitters and inflammatory mediators. However, it h...

Abstract IgE mediated mast cell activation is a key feature of allergic disease, although the mechanisms that govern homeostasis are not fully understood. The TGFb signaling pathway has been shown to regulate effector function. Furthermore, variants in associated with diseases; regulation likely involved allergy diathesis. Patients Loeys Dietz Syndrome (LDS), disorder caused by loss function TG...