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The route of administration of DNA vaccines can play a key role in the magnitude and quality of the immune response triggered after their administration. DNA vaccines containing the gene of the membrane-anchored glycoprotein (gpG) of the fish rhabdoviruses infectious haematopoietic necrosis virus (IHNV) or viral haematopoietic septicaemia virus (VHSV), perhaps the most effective DNA vaccines ge...

In March 1984, a new rhabdovlrus was isolated from moribund cultured hlrame (Japanese flounder) Parahchthys ohvaceus and from ayu Plecoglossus alhvelis fry In Hyogo Prefecture, Japan. From February through May 1985, the virus was again isolated from hirame in seawater tanks in Hyogo and Kagawa Prefecture, and in Hokkaldo, Japan. At temperatures between 5 and 20 "C the vlrus replicated and induc...

Deoxyribonucleic acid (DNA) vaccination is based on the administration of the gene encoding the vaccine antigen, rather than the antigen itself. Subsequent expression of the antigen by cells in the vaccinated hosts triggers the host immune system. Among the many experimental DNA vaccines tested in various animal species as well as in humans, the vaccines against rhabdovirus diseases in fish hav...

—Monoclonal antibodies (MAbs) directed against the snakehead rhabdovirus (SHRV) were produced. These MAbs were characterized by immunofluorescence and neutralization tests, and by their ability to immunoprecipitate viral proteins. Of 15 MAbs developed, 9 were isotyped as IgGl and 6 were IgG2a. Eight of the MAbs recognized the viral glycoprotein in an immuno precipitation assay. Three of these, ...

To assess the risk posed by vertical transmission of viral haemorrhagic septicaemia virus, VHSV, in rainbow trout ova, an experimental challenge study was conducted. Prior to the fertilisation process samples of milt and/or ova were infected with VHSV. Virus was not detected from either the fertilised ova or hatched fry. These results indicate that the risk associated with VHSV vertical transmi...

Cell culture for amplification and the techniques most used for identification of salmonid viruses neutralization, immunofluorescence and, to a lesser extent, immunoperoxldase, complen~ent fixation, agglutination, electron microscopy, immunodifussion or radioimmunoassay may soon be replaced by other techniques such as enzyme immunoassays (immunodot and enzyme-linked immunosorbent assay, ELISA) ...