A procedure for cryogenic storage of alkaloid producing cell lines of periwinkle, Catharanthus roseus (L.) G. Don., has been developed. The procedure differs from established cryopreservation protocols in several aspects. Specifically, 4-day-old suspension subcultures of three cell lines were precultured in nutrient media supplemented with 1 molar sorbitol for 6 to 20 hours. The cells were then...

We present a new perfusion technique that allows arteries down to the level of capillaries to be fixed while relaxed and under a known intravascular pressure. Through a catheter inserted into the right renal artery of 12-week-old male spontaneously hypertensive rats (n = 9) and control Wistar-Kyoto rats (n = 11), the kidney vessels were rinsed with human plasma, relaxed by papaverine, and perfu...

BACKGROUND Different environmental conditions, such as high temperature or exposure to some chemical agents, may affect the force decay of different methods of space closure during orthodontic treatment. The aim of this in vitro study was to evaluate the force decay pattern in the presence of tea as a popular drink in some parts of the world and two mouthwashes that are usually prescribed by th...

This study reviewed the potential for survival of a pathogenic bacteria when inoculated into McCarey-Kaufman modified tissue culture media 199. A clinically isolated specimen of Staphylococcus aureus was selected and rabbit eyes inoculated with 0.1 ml. of the organism in a suspension of 1 to 3 x 10(-5). Upon enucleation 12 hours later no clinical signs of infection were noted. Limbal-conjunctiv...

 Abstract— Immersion is a widely valued experience when playing digital games however it is only one component of the gaming experience. In this chapter, we review our specific approach to immersion in relation to other concepts that are used to describe gaming experiences. These include: concepts that are not specific to games such as flow and attention; generic conceptualizations of the gami...

Pistachio green kernel, is obtained by removing the red testa of around the whole kernel. In this study, the suitable peeling conditions for removing the testa of the kernel were determined using hot water in five termperature levels of 75, 80, 85, 90, and 95°C and diffetent hot water immersion times of 2, 4, 6, 8, and 10 minutes in riped and unripe samples. Quality factors including the number...

Background: Calcium phosphates (CPs) form hydroxyapatite (HA) in physiological solutions. These are commonly used to treat dentine hypersensitivity (DH) as they mimic the mineral composition of natural tooth. Aim: The present study aims characterise apatite formation ability a commercially available calcium phosphate TeethmateTM (TM) physiological-like media. Materials and Methods: In this stud...

Aim: The aim of this study was to evaluate the influence of acrylic resin immersion in different mouthwashes on hardness, roughness and color. Methods: Specimens of an orthodontic selfcured acrylic resin (Orto Clas®) were produced and immersed in five mouthwashes: Plax® Classic (Colgate®); Plax® alcohol-free (Colgate®); Listerine® (Johnson & Johnson®); Periogard® (Colgate®) and Periogard® alcoh...

The objective of the study was to determine the influence of simulated microgravity by exposure to dry immersion on the craniomandibular system. Twelve healthy male volunteers participated in a 3-day dry immersion study. Before and immediately after exposure we measured maximal bite force using piezoresistive sensors. The mechanical properties of the jaw and cervical muscles were evaluated befo...

The main aim of the present in-vitro study is to assess effect daily consumable drinks on aesthetics dental composite resin materials. Sixty-four microhybrid and nanohybrid direct (3M™ Filtek™ Z350 XT Universal Restorative, Bulk Fill Flowable USA) block samples were made with a dimension 15mm X 2mm are allocated into four groups. Each sample was immersed in test media such as Arabic coffee, bla...