A monoclonal antibody was used to prepare immunoaffinity columns that efficiently bind monoamine oxidase B activity but not monoamine oxidase A activity from detergent extracts of human liver mitochondria. The only discrete polypeptide component that eluted from affinity columns with potassium thiocyanate migrated in sodium dodecyl sulfate-polyacrylamide gels with an apparent molecular weight o...

Due to the widespread use of dexamethasone in racing horses, mostly in low doses by intra-articular administration for the treatment of inflammatory processes, a method is developed to detect this drug in horse urine samples using liquid-liquid extraction followed by immunoaffinity chromatography. Liquid chromatography with diode-array detection is used for the identification of the drug. The u...

INTRODUCTION Knowledge of the composition of protein complexes provides key insights into their functions. Immunoaffinity purification provides an effective means for isolating protein complexes and elucidating their composition. Immunoisolation is achieved with antibodies directed either specifically against the proteins of interest or against tags that are coupled to the proteins of interest....

Cholera enterotoxin (CT) and heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli share a common antigenic determinant. In addition, CT and LT each have a unique antigenic determinant. Antisera were prepared by immunoaffinity chromatography against these unique antigenic determinants, that is, antiserum that reacted with CT but not with LT and antiserum that reacted with LT...

INTRODUCTION Knowledge of the composition of protein complexes provides key insights into their functions. Immunoaffinity purification provides an effective means for isolating protein complexes and elucidating their composition. Immunoisolation is achieved with antibodies directed either specifically against the proteins of interest or against tags that are coupled to the proteins of interest....

The plasma of patients with myeloproliferative diseases was examined by polyethylene glycol (PEG) precipitation, analytical ultracentrifugation, and immunoaffinity chromatography for the presence of high molecular weight complexes of IgG and fibronectin. Abnormal circulating high molecular weight material was identified by ultracentrifugation in all patients. This was precipitated by PEG and wa...

A newly developed system for defining watertight volume geometry on imported foreign CAD models is described. This system is based on an overlay grid mesher that is being developed in the GAMBIT preprocessing code. It is being explored as an alternative way of cleaning dirty geometries. This overlay meshing based geometry cleanup enables an automatic volume reconstruction from inconsistent B-Re...

A liquid chromatographic method with comfirmation by capillary electrophoresis was used to determine 12 sulfonylurea herbicides in agricultural water. Analysis of 3 different water matrixes fortified at 2 levels gave good recoveries with adequate sensitivity at the 0.1 ppb level. A portion of the water was acidified with acetic acid and loaded onto an RP-102 solid-phase extraction (SPE) cartrid...