A quantitative assay for lymphocytic choriomeningitis virus was developed and standardized. The assay is based on direct immunofluorescent staining of infected L-929 cell monolayers and enumeration of cells containing fluorescent viral antigens. Maximal adsorption of virus to cells occurred within 1 h. Observations on the sequential development of viral antigens within cells showed that specifi...

On the cover: Dynamic change of the localization of FROUNT and CCR5 in CCL4-stimulated cell. Immunofluorescent staining at 20 min after stimulation detects colocalization of FROUNT (red) and CCR5 (green) in both the pseudopodial area and the inner cellular space (colocalization is white). Toda, E., Y. Terashima, T. Sato, K. Hirose, S. Kanegasaki, and K. Matsushima. 2009. FROUNT is a common regu...

An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were prospectively compared with the culture results for 776 specimens from patients with suspected melioidosis. The sensitivities of both IF tests were 66%, and the specificities were 99.5 and 99.4%, respect...

Serum containing a monoclonal IgM protein from a patient with Waldenstroms' macroglobulinaemia gave intense immunofluorescent staining of kidney nuclei. The Fab mu fragments of this immunoglobulin were obtained. The IgM and Fab fragments reacted in vitro with kidney nuclei using unfixed cryostat sections of rat or mouse kidney. After treatment of the patient with chemotherapy, the monoclonal Ig...

The extent and significance of renal biopsy abnormalities in patients with systemic lupus erythematosus (SLE) without clinical renal abnormalities is controversial. We report 11 consecutive SLE patients who were biopsied without clinical renal abnormalities. All 11 patients had mesangial changes either by light microscopy or by immunofluorescent staining, and none had changes of focal or diffus...

Transcriptional profiling is a key technique in the study of cell biology that is limited by the availability of reagents to uniquely identify specific cell types and isolate high quality RNA from them. We report a Method for Analyzing RNA following Intracellular Sorting (MARIS) that generates high quality RNA for transcriptome profiling following cellular fixation, intracellular immunofluoresc...

towelve calves of 4-10 months old with clinical signs suspected to bovine viral diarrhea virus (bvdv) infection were selected for this study. histopathologic sections were performed on formalin fixed paraffin-embded ear notch biopsies, mounted on poly-l-lysine coated slides and stained for bvdv by anti-bvdv monoclonal antibody labelled with fluorescein isothiocyanate. stained sections were exam...

there are many effective chemothereutic agents used in influenza disease which some of them inhibit virus replication by interfering with fluv (influenza virus) viral binding or its penetration into cell membrane. a series of polyoxometalates compounds such as pom-523 and pm-504 have been synthesized and have showed inhibitory effects on viruses. in this study we examined anti influenza activit...

RATIONALE Circulating levels of endothelin (ET)-1 and endogenous ET(A)-mediated constriction are increased in human aging. The mechanisms responsible are not known. OBJECTIVE Investigate the storage, release, and activity of ET-1 system in arteries from young and aged Fischer-344 rats. METHODS AND RESULTS After NO synthase inhibition (L-NAME), thrombin contracted aged arteries, which was in...

AIM To investigate histologic abnormalities in the gastric smooth muscle of patients with diabetes mellitus (DM). METHODS Full-thickness gastric specimens were obtained from patients undergoing surgery for gastric cancer. H&E stain and Masson's Trichrome stain were performed to assess the degree of fibrosis. Immunohistochemical staining using various antibodies was also performed [antibodies ...