The aim of this study was to identify a suitable method for detecting lymph node metastasis of gastric cancer (GCA) by hematoxylin and eosin (HE) and immunohistochemical (IHC) staining. We investigated lymph node metastasis using pan-cytokeratin (CK) and epithelial membrane antigen (EMA) IHC staining in a total of 1,422 lymph nodes from 100 patients who underwent radical gastrectomy between 200...

BACKGROUND Prostate cancer diagnosis is routinely made by the histopathological examination of formalin fixed needle biopsy specimens. Frequently this is the only cancer tissue available from the patient for the analysis of diagnostic and prognostic biomarkers. There is, therefore, an urgent need for methods that allow the high-throughput analysis of these biopsy samples using immunohistochemic...

BACKGROUND The effectiveness of photodynamic therapy (PDT) for ablation of high grade dysplasia (HGD) in Barrett's esophagus (BE) is typically reported histologically. Following successful PDT, Barrett's mucosa is replaced with neosquamous mucosa. The objective of this study was to compare the expression of p53 protein in neosquamous mucosa as compared to that in HGD samples not treated with PD...

Rocky Mountain spotted fever (RMSF) is the most severe tickborne infection in the United States and is a nationally notifiable disease. Since 1981, the annual case-fatality ratio for RMSF has been determined from laboratory-confirmed cases reported to the Centers for Disease Control and Prevention (CDC). Herein, a description is given of patients with fatal, serologically unconfirmed RMSF for w...

OBJECTIVES Neuroendocrine neoplasms (NENs) are heterogeneous neoplasms, which are sometimes malignant, although predicting metastasis is difficult. INSM1 is a transcription factor expressed transiently in embryonic neuroendocrine (NE) tissue, thought to coordinate termination of cell division with differentiation of NE and neuroepithelial cells. In adult tissues, INSM1 has been identified in mu...

OBJECTIVES To detect amplification of the HER2/neu gene by means of fluorescence in situ hybridization (FISH) in a series of 13 salivary duct carcinomas (SDCs) and to compare the results with immunohistochemical (IHC) assessment of HER2/neu protein expression. DESIGN Retrospective analysis. SETTING Department of Pathology, University of Brescia. PATIENTS We studied 13 cases of SDC diagnos...

This study aims to examine the effect of temperature and duration pasteurization exposure on osteosarcoma tumor tissue degeneration, necrosis, cell viability. Using tissue, researchers evaluated viability following pasteurization. Hematoxylin-eosin (HE) staining was utilized assess degeneration whereas CKAP4 immunohistochemical (IHC) The Kruskal-Wallis test showed a significant increase in dege...

Context.— The Ventana programmed death ligand-1 (PD-L1) SP142 immunohistochemical assay (IHC) is approved by the US Food and Drug Administration as companion diagnostic to identify patients with locally advanced or metastatic triple-negative breast cancer for immunotherapy atezolizumab, a monoclonal antibody targeting PD-L1. Objective.— To determine interobserver variability in PD-L1 IHC interp...

Immunohistochemical (IHC) analysis of tissue biopsies is currently used for clinical screening solid cancers to assess protein expression. The large amount image data produced from these samples requires specialized computational pathology methods perform integrative analysis. Even though proteins are traditionally studied independently, the study co-expression may offer new insights towards pa...