An automated solid phase extraction (SPE) protocol to determine carbamazepine in human serum has been developed and validated using a simple, rabid sensitive liquid chromatography-based bio-analytical method. Extraction of was carried out an on-line SPE tool short protein-coated (PC) ODS silica pre-column (PC-ODS-pre-column) phosphate buffer saline (PBS) with pH 7.4 as solvent. There are two di...

Artemisinin extracted from Artemisia annua L. is the best medicine with the highest efficiency, the most effective and the lowest toxicity in treating ague nowadays. At present, most studies focus on artemisinin and its derivatives, while the study and report about the other active components are rare. This paper purposed to further discover new indication of Artemisia annua L. connecting with ...

Genistein, an isoflavone, has been demonstrated to promote the health of human beings by reducing the incidence of specific chronic diseases, namely, cancer and atherosclerosis. The present investigation explores a novel method of extraction of genistein from soy source which consists of a bioconversion reaction using fermentation by microorganism namely Streptomyces roseolus NRRL B-5424. In si...

A key unmet need in metabolomics is the ability to efficiently quantify a large number of known cellular metabolites. Here we present a liquid chromatography (LC)-electrospray ionization tandem mass spectrometry (ESI-MS/MS) method for reliable measurement of 141 metabolites, including components of central carbon, amino acid, and nucleotide metabolism. The selected LC approach, hydrophilic inte...

Natural products have coming from various source materials including terrestrial plants, terrestrial microorganisms, marine organisms, terrestrial vertebrates and invertebrates have importance as they provide an amazing source of new drugs as well as new drug leads and new chemical entities for further drug development (McCurdy & Scully, 2005; Chin et al., 2006). Morphine, vincristine, codeine,...