We aim to develop an in situ microfluidic biosensor based on laccase from Trametes pubescens with flow-injection and amperometry as the transducer method. The enzyme was directly immobilized by potential step chronoamperometry, and the immobilization was studied using cyclic voltammetry and electrochemical impedance spectroscopy. The electrode response by amperometry was probed using ABTS and s...

This study aimed to prepare robust immobilized formate dehydrogenase (FDH) preparations which can be used as effective biocatalysts along with functional oxidoreductases, in which in situ regeneration of NADH is required. For this purpose, Candida methylica FDH was covalently immobilized onto Immobead 150 support (FDHI150), Immobead 150 support modified with ethylenediamine and then activated w...

In this paper, based on the previous steps, a facile in situ reduction method was developed to controllably prepare polystyrene/Ag (PS/Ag) core-shell-shaped nanostructures. The crucial procedure includes surface treatment of polystyrene core particles by cationic polyelectrolyte polyethyleneimine, in situ formation of Ag nanoparticles, and immobilization of the Ag nanoparticles onto the surface...

Sexual behavior in males is vulnerable to stress and it has been suggested that alterations in sexual behavior during stress is concomitant with spermatogenesis dysfunction. In this study, we investigated the effects of immobilization on sexual behavior and whether or not these effects are accompanied by changes in spermatogenesis process. The effect of antioxidant treatment on the sexual behav...

soil affected by salt (nacl) is a major problem worldwide and in areas with potential agriculture; lands in many countries are not enough to support crop production. the development of salt tolerant cultivars would be enhanced by better understanding of the genetic control of tolerance to salt stress. a new cereal, tritipyrum, a range of amphiploids between triticum spp. and thinopyrum spp....

natural radionuclides present in soil as well as certain anthropogenic radionuclides released to the environment are the major contributors to terrestrial outdoor exposures. in the assessment of human exposures from environmental radioactivity, besides the conventional method of soil and vegetation sampling combined with laboratory based analyses of environmental media, the other choice would b...