In vitro maturation (IVM) is emerging as a popular technology at the forefront of fertility treatment and preservation. However, standard in vitro culture conditions usually increase reactive oxygen species (ROS), which have been implicated as one of the major causes for reduced embryonic developmental competence. It is well-known that higher than physiological levels of ROS triggers granulosa ...

Objective: Alchornea floribunda leaves are widely used in ethnomedicine for the management of immuno-inflammatory disorders. We investigated the in vivo and in vitro antioxidant activity of the leaf extract, fractions and isolated compounds of A. floribunda.Materials and Methods: The ethyl acetate fraction of the methanol leaf extract was subjected to several chromatographic separations to isol...

Purified serum paraoxonase (PON1) had been shown to attenuate the oxidation of LDL in vitro. We critically reevaluated the antioxidant properties of serum PON1 in the in vitro assays initiated with copper or the free radical generator 2,2'-azobis-2-amidinopropane hydrochloride (AAPH). The antioxidant activity of different purified PON1 preparations did not correlate with their arylesterase (AE)...

Haptoglobin (Hp) is a mammalian serum glycoprotein showing a genetic polymorphism with three types, 1-1, 2-2 and 1-2. Hp appears to conserve the recycling of heme-iron by forming an essentially irreversible but non-covalent complex with hemoglobin which is released into the plasma by erythrocyte lysis. As an important consequence, Haptoglobin-Hemoglobin complex (Hp-Hb) shows considerable antiox...

Objective: The purpose of the current study was to investigate the in vivo (analgesic, antidiarrheal, neurological, and cytotoxic) and in vitro (antioxidant, antimicrobial, thrombolytic and anthelmintic) activity of different fractions of methanolic extract of Momordica charantia. Materials and Methods: The antioxidant property ...

in this study, we examined the antioxidant activities of methanolic extract of three endemic species of salvia from iran (s. lachnocalyx, s. reuterana and s. sahendica) employing various established in vitro systems including ferric reducing antioxidant power, trolox equivalent antioxidant capacity assay and scavenging of 1,1-diphenyl-2-picrylhydrazyl radical. the results revealed that s. reute...

In this investigation, the In-vitro antioxidant Activity of extracts leaves Ougeinia oojeinensis was evaluated. The activity in vitro measured by means DPPH, Reducing power and hydroxyl radical scavenging activity. DPPH ethyl acetate extract exhibited percent inhibition 66.774% its IC50 value found to be 43.503μg/ml. methanol 76.267% 25.722μg/ml. Ascorbic acid used as a reference compound which...

Lantadenes are pentacyclic triterpenoids present in the leaves of the plant Lantana camara. In the present study, in vitro antioxidant activity and free radical scavenging capacity of lantadene A was evaluated using established in vitro models such as ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picryl-hydrazyl (DPPH•), hydroxyl radical (OH•), nitric oxide radical (NO•), superoxide ...

The genus Verbascum L. belongs to the family Scrophulariaceae and includes plants that have been used widely in traditional medicine for a long time. Methanolic extracts of three Verbascum species from flora of Iran were in vitro screened for their possible antioxidant activities by three complementary test systems, namely DPPH free radical-scavenging, metal chelating activity and β-carotene/li...

the antioxidant activities of methanol (me), ethanol (ee), water (we) and ethyl acetate (eae) extracts of four inula helenium l. taxa (i. helenium ssp. orygalis (boiss.) grierson, i. helenium ssp. pseudohelenium grierson, i. helenium ssp. turcarasemosa grierson and i. helenium ssp. vanensis grierson) were investigated. the phosphomolybdenum assay, β-carotene–linoleate bleaching and dpph radical...