objective(s):cutaneous leishmaniasis is a common and endemic disease in khorasan province in north-east of iran. the pentavalant antimony (sb v) is the mainstay of treatment that has many side effects and resistance to the drug has been reported. the microbicidal effect of ozone was proven in different microorganisms. since there is no study in this respect and to achieve a low cost and effecti...

the objective of the present study was to investigate the effect of different doses of some 7 natural semi-arid medicinal plants’ essential oils on in vitro ruminal digestion and fermentation patterns of a mid-forage (alfalfa hay: concentrates, 1:1) diet. treatments consisted of either basal diet alone (control) or added with 35, 70, 140 or 280 µl l-1 of coriander, oregano, caraway, cumin, cinn...

in order to determine the most effective hormones on the in vitro oocyte maturation (germinal vesicle breakdown - gvbd) in a hybrid sturgeon bester, ovarian follicles were incubated at the migratory nucleus stage in the presence of several steroids, gonadotropin preparations and forskolin. occurrence of gvbd and 17,20β-dihydroxy-4-pregnen-3 -one (dhp) production were followed during an expe...

the aim of the present study was to investigate the effect of follicle stimulating hormone (fsh) on survival, activation and growth of ovine primordial follicles using histological studies. pieces of ovine ovarian cortex were obtained and cultured for 6 days in minimum essential medium supplemented with or without fsh (50 ng/ml). these fragments were then process to be used for histology compar...

plantlets grown in conventional tissue culture systems usually encounter physiological and anatomical abnormalities including inability to photosynthesize, low chlorophyll content, open stomata, lack of a cuticle layer in the leaf, abnormal xylem parenchyma etc. photoautotrophic and photomixotrophic systems could diminish these problems. the purpose of this study was to increase the chlorophyll...

In vitro culture systems using bone marrow cells from BALB/C mice were set up in medium supplemented with spleen-derived medium. Bone marrow cells grown in spleen-derived medium gave rise to cultures containing >97% mast cells. The cells were used in polarisation chemotaxis assays with the intention of determining the effect of nerve tissue-derived neurotransmitters on mast cell migration. ...