Background: Hyaline cartilage defects exhibit a major challenge in the field of orthopedic surgery owing to its limited repair capacity. On the other hand, mesenchymal stem cells (MSCs) are regarded as potent cells with a property of cartilage regeneration. We aimed to optimize marrow-derived MSC chondrogenic culture using a small bioactive molecule referred to as BIO. Methods: MSCs from the ma...

To determine the pharmacological (Anti-inflammatory) potential of in-vitro cultures of Asparagus racemosus. Anti-inflammatory activity of methanolic extracts of shoot, root their calli and genetically transformed shoot culture was performed using carrageenan induced rat paw edema method. The albino rats were treated orally with two dosage (500 or 1000 mg/kg body weight of rats) of drugs, one ho...

The studies conducted to determine the stage of its usability in prospective fertility restoration testicular tissues taken from prepubertal patients undergoing oncology treatment were screened. In addition, current status spermatogonial stem cell cultures, tissue and organoid research their potential examined.
 Spermatogonial cells are only found tissue. Germinal serial not found. Since s...

In vitro culture systems using bone marrow cells from BALB/C mice were set up in medium supplemented with spleen-derived medium. Bone marrow cells grown in spleen-derived medium gave rise to cultures containing >97% mast cells. The cells were used in polarisation chemotaxis assays with the intention of determining the effect of nerve tissue-derived neurotransmitters on mast cell migration. ...

background: promastigotes of leishmania spp. have been readily cultured, but the axenic culture of amastigotes has been successful in l. donovani , l .infantum l. mexicana and l. pifanoi . however, some species such as l. major, is much less amenable to axenic cultivation. in present study, we describe an in vitro culture system for the generation and propagation of axenic amastigotes form of l...

objective: the present study is aimed to investigate in vitro thrombolytic activity of three bangladeshi medicinal plants averrhoa bilimbi (oxalidiaceae), clerodendrum viscosum (verbanaceae) and drynaria quercifolia (polypodiaceae). materials and methods: each the plant was extracted with methanol at room temperature and the concentrated methanolic extracts (mef) were fractionated by the modifi...

background: pentavalent antimonials are still the first choice treatment for leishmaniasis, but with low efficacy and resistance is emerging. in the present study, the effect of meglumine antimoniate (ma, glucantime) combined with paromomy-cin, miltefosine or allopurinol on in vitro susceptibility of leishmania tropica resistant isolate was evaluated. method : the drugs were obtained from comme...