background: repeated in vitro fertilization (ivf) failure together with ovarian hyperstimulation syndrome (ohss) is one of the distressing situations leading couples to search for alternative treatment options. for such patients with polycystic ovarian syndrome (pcos) who have experienced ovarian hyperstimulation syndrome, mild ovarian stimulation with in vitro oocyte maturation could be a prom...

background: leukemia inhibitory factor (lif) is a 45-56 kda glycoprotein that has an important role in proliferation and embryo implantation. its effect on oocyte maturation and how to exert the function remained to be elucidated. methods: immature mice superovulated with human menopausal gonadotropin and germinal vesicle (gv) oocytes were obtained from ovary 48 hours after. gv oocytes were cul...

abstract: introduction & objective: the infertility treatment by using in vitro matured oocyte has many potential applications. it minimizes the risk of ovarian hyper stimulation and is an alternative treatment for women with polycystic ovarian syndrome who may have problems regarding stimulation for ivf. this method may prove important for subjects in need of fertility preservation and also pr...

The present study examined the kinetics of glutathione (GSH) concentration during maturation and after fertilization in pig oocytes and its relevance to the ability of pig oocytes to form a male pronucleus after in vitro fertilization. The GSH concentration was significantly higher in pig oocytes matured in Waymouth medium than in pig oocytes matured in either modified (m) TCM-199 or mTLP media...

Polyspermy is fairly common during porcine in vitro fertilisation (IVF), perhaps due to incomplete in vitro oocyte maturation (IVM). Porcine cumulus cells (CCs) layered around the oocyte produce large amounts of extracellular hyaluronan (HA) when forming an expanding cell cloud during the last phase of oocyte maturation. The specific actions of HA are mediated via HA-binding proteins (HABPs), s...

The objective of this study was to investigate the influence of various factors on blastocyst development in a bovine in vitro embryo production system. Two production systems were originally compared: Protocol 1, which utilized oocyte recovery by slicing, 20 % fetal bovine serum with FSH (FBS+) for oocyte maturation, and sperm preparation system 1 (F1), and Protocol 2, which utilized oocyte re...

BACKGROUND Assisted reproductive technologies allow to utilize a limited number of fully grown oocytes despite the presence in the ovary of a large pool of meiotically incompetent gametes potentially able to produce live births. In vitro folliculogenesis could be useful to recruit these oocytes by promoting their growth and differentiation. METHODOLOGY/PRINCIPAL FINDINGS In vitro folliculogen...

4 Introduction 4 A. Zebrafish as a Model Organism for Developmental Biology 4 B. Processes of Oocyte Maturation in Zebrafish 5 C. Wnt Signaling Pathways and Their Possible Role in Oocyte Maturation 11 D. Preliminary Findings and Objectives of This Research Project 15 Materials and Methods 16 A. Overview 16 B. Oocyte Harvesting and in vitro Maturation 17 C. RNA Extraction and cDNA Conversion 18 ...