Background: Cryopreservation of in vitro matured oocytes is a useful technique because the oocytes can be used for some assisted reproductive technologies. On the other hand, the cryopreservation of oocytes is an open problem as a result of their structural sensitivity to the freezing process. The purpose of this study was to evaluate the effect of vitrification on in vitro development of vitri...

Isolated nucleoli from rat Novikoff hepatoma ascites cells incorporated 32P of [T-~~P]ATP into acid-soluble nucleolar proteins in vitro in systems containing 0.25 M sucrose, 5 mM MgCL, 12.5 mM NaCl, and 0.05 M Tris-HCl buffer (pH 7.3) at 37”. Although the total uptake of 32P reached a maximum after 15 min of incubation, the uptake into some protein bands increased with longer incubation times. ...

Diabetes mellitus (DM) causes the development of a specific cardiomyopathy that results from the metabolic derangements present in DM and manifests as cardiac contractile dysfunction. Although myocardial dysfunction in Type 1 DM has been associated with defects in the function and regulation of the sarcoplasmic reticulum (SR), very little is known about SR function in Type 2 DM. Accordingly, th...

Aluminium is well known to inhibit plant elongation, but the role in this inhibition played by water relations remains unclear. To investigate this, tobacco (Nicotiana tabacum L.) suspension-cultured cells (line SL) was used, treating them with aluminium (50 microM) in a medium containing calcium, sucrose, and MES (pH 5.0). Over an 18 h treatment period, aluminium inhibited the increase in fres...

We have previously described in rats the selective uptake of HDL-associated cholesterol esters (traced by [3H]cholesteryl oleyl ether) in excess of the uptake of HDL-associated apoA-I. In the present studies we show that the mechanism also exists in cultured cells of human and mouse origin as well. This selective uptake represents a net uptake of cholesterol esters and not an isotope exchange, ...

five different media with two concentrations and kinds of carbohydrates and peptone treatments were examined for their effectiveness in promoting asymbiotic seed germination of epipactis  veratrifolia. results revealed significant differences between media on seed germination percentage and protocorm growth and fast medium has the best effect on both. carbohydrate treatments had significant eff...

An efficient and cost effective protocol for in vitro propagation of Pineapple (Ananas comosus var. Queen) has been developed. In the proliferation stage, agar based Murashige and Skoog (MS) media was supplemented with 3.0 mg/l benzyleaminopurine (BAP), 0.5 mg/l indole acetic acid (IAA) and 50 mg/l adenine sulphate as RBC design experiment. Two approaches were taken to reduce the chemical cost ...

In a four-year experiment, five nitrogen rates (0, 60, 120, 180 and 240 kg N ha-1) were tested over irrigated sugar beets grown on clays, underMediterranean conditions, in centralGreece. There, sugar beets are commonly grown under water shortages, high temperatures and high soil Na concentrations. Contrary to previous reports, N rates did not affect significantly population density (as asse...