This paper proposes a novel approach for the automatic detection of allergy test (allergy lesion). A hyperspectral microscope system was used to image the test samples which were diagnosed by dermatologist. It was found that allergy of different levels, and healthy skin cells show absorption spectra, which are sufficiently characteristic and yet reproducible enough to allow for differentiation ...

A high speed swept source optical coherence tomography (SS-OCT) system capable of full-range imaging is presented. Wave-number carrier frequency is introduced into the spectral interference signal by a transmissive dispersive optical delay line (TDODL). High carrier frequency in the spectral interference signal corresponding to an equivalent distance-shift is exploited to obtain full-range OCT ...

Photoacoustic Computed Microscopy (PACM) is a new label-free microscopic method that combines current photoacoustic microscopy technique with a modelbased inverse reconstruction algorithm to provide functional images of microvasculature. This article demonstrates its in vivo imaging ability of quantifying important functional parameters at the small vessel level in a rodent model. This new tech...

A novel fluorescent probe for in vitro and in vivo imaging of dipeptidyl peptidase-4 (DPP-4) was designed and synthesized. This probe is successfully utilized to screen DPP-4 inhibitors in living 3T3-L1 cells and zebrafish, which provides a novel approach for the discovery of anti-diabetes drugs.

It is important to develop a noninvasive label-free detection method to monitor dynamic phenomena in biology and medicine. Here, we utilize nanoplasmonic optical imaging as the noninvasive and label-free method in order to monitor in vitro amyloid fibrogenesis in real-time, which is considered as the primary pathological mechanism of Alzheimer's disease. Using Rayleigh scattering of gold nanopl...

Two-photon microscopy has been used to perform high spatial resolution imaging of spine plasticity in the intact neocortex of living mice. Multiphoton absorption has also been used as a tool for the selective disruption of cellular structures in living cells and simple organisms. In this work, we exploit the spatial localization of multiphoton excitation to perform selective lesions on the neur...

As molecular imaging moves towards lower detection limits, the elimination of endogenous background signals becomes imperative. We present a facile background-suppression technique that specifically segregates the signal from surface-enhanced Raman scattering (SERS)-active nanoparticles (NPs) from the tissue autofluorescence background in vivo. SERS NPs have extremely narrow spectral peaks that...

In the last decade, intravital microscopy of breast tumors in mice and rats at single-cell resolution has resulted in important insights into mechanisms of metastatic behavior such as migration, invasion and intravasation of tumor cells, angiogenesis, and immune cells response. We have recently reported a technique to image orthotopic mammary carcinomas over multiple intravital imaging sessions...

Chronic in vivo imaging and electrophysiology are important for better understanding of neural functions and circuits. We introduce the new cranial window using soft, penetrable, elastic, and transparent, silicone-based polydimethylsiloxane (PDMS) as a substitute for the skull and dura in both rats and mice. The PDMS can be readily tailored to any size and shape to cover large brain area. Clear...

Time-lapse imaging is a technique that allows for the direct observation of the process of morphogenesis, or the generation of shape. Due to their optical clarity and amenability to genetic manipulation, the zebrafish embryo has become a popular model organism with which to perform time-lapse analysis of morphogenesis in living embryos. Confocal imaging of a live zebrafish embryo requires that ...