Coons and Kaplan (1950) developed a rapid, sensitive, and specific fluorescent antibody technique for staining viruses in tissue sections. They used a direct staining procedure in which a specific fluorescent antibody preparation is applied directly to the cell being studied. In the indirect procedure (Weller and Coons, 1954), the virus is first reacted with a specific nonfluorescent antiserum ...

We compared histological and immunological techniques in the early diagnosis of Pneumocystis carinii pneumonia in bronchial lavage fluid of steroid-treated rats. The rats were sacrificed weekly and lavage fluids were: (i) examined with cresyl echt violet and Giemsa stains; (ii) examined for P. carinii antigens by indirect fluorescent-antibody, counterimmunoelectrophoresis, and double-diffusion ...

We present a methodology for extracting the vascular network in the human retina using Dijkstra's shortest-path algorithm. Our method preserves vessel thickness, requires no manual intervention, and follows vessel branching naturally and efficiently. To test our method, we constructed a retinal video indirect ophthalmoscopy (VIO) image database from pediatric patients and compared the segmentat...

Moody, Max D. (Communicable Disease Center, Atlanta, Ga.) and Wallis L. Jones. Identification of Corynebacterium diphtheriae with fluorescent antibacterial reagents. J. Bacteriol. 86:285-293. 1963.-Conditions are described whereby fluorescent-antibody reagents could be prepared and used to identify Corynebacterium diphtheriae in pure and mixed cultures and in clinical materials. The use of O an...

A method of fluorescent nanoparticle-based indirect immunofluorescence microscopy (FNP-IIFM) was developed for the rapid detection of Mycobacterium tuberculosis. An anti-Mycobacterium tuberculosis antibody was used as primary antibody to recognize Mycobacterium tuberculosis, and then an antibody binding protein (Protein A) labeled with Tris(2,2-bipyridyl)dichlororuthenium(II) hexahydrate (RuBpy...

A simple hemagglutination inhibition (HI) test for the serological diagnosis of toxoplasmosis has been developed and evaluated. A total of 84 human and 120 mouse serum samples were tested by the newly developed HI test and compared with an immunoglobulin G-indirect fluorescent antibody test. Statistical analysis of serum titers obtained by using the HI test and the immunoglobulin G-indirect flu...

Determining total energy expenditure (EE) in children under free-living conditions has become of increasingly clinical interest. The aim of this study was to compare three different methods to assess EE triaxial accelerometry (TriTrac-R3D; Professional Products, Division of Reining International, Madison, WI, USA), activity diary and heart-rate (HR) monitoring combined with indirect calorimetry...

mycoplasma genitalium (m.genitalium) is a sexually transmitted pathogen. detection of this microorganism in clinical specimens by culture is rather difficult and time consuming. the aim of this study was to produce polyclonal antibody against a synthetic peptide from p110 protein of m.genitalium in order to develop a diagnostic tool for detection of this microorganism in clinical specimens. a s...

Three commercially available assays (the Varicelisa Test Kit [Whittaker M.A. Bioproducts, Walkersville, Md.], the VZV Indirect Fluorescent-Antibody Test [Electro-Nucleonics, Inc., Columbia, Md.], and the Litton VZV Bio-EnzaBead Screen Kit [Litton Bionetics, Inc., Charleston, S.C.]) and two enzyme-linked immunosorbent assays used in our laboratory, one using a membrane-associated antigen and the...

Mice infected neonatally with homogenates from lymphomas induced by the Moloney virus showed a deficient or delayed antibody response, measured by the indirect fluorescent antibody test against Moloney lymphoma target cells in com parison with mice in.fected as adults. In neonatally infected mice there was a corre lation between the development of antibodies and the length of the preleukemic la...