نتایج جستجو برای: insect cell

تعداد نتایج: 1718296  

Fatemeh Mohammadkhan Ramin Mazaheri Nezhad Fard, Seyed Milad Vahedi

Haemoplasmas contain Eperythrozoon and Haemobartonella species, which are widespread causes of animal and human infections. These obligatory cell parasites were previously known as Hemobartonella; now hemotropic mycoplasmas. These bacteria are identified by the lack of cell wall and their small genome. The usual diameter is between 0.3 and1μm. Mycoplasmasarepolymorphi...

2015
Maren Bleckmann Markus H.-Y. Fritz Sabin Bhuju Michael Jarek Margitta Schürig Robert Geffers Vladimir Benes Hüseyin Besir Joop van den Heuvel Yi Li

The Baculoviral Expression Vector System (BEVS) is the most commonly used method for high expression of recombinant protein in insect cells. Nevertheless, expression of some target proteins--especially those entering the secretory pathway--provides a severe challenge for the baculovirus infected insect cells, due to the reorganisation of intracellular compounds upon viral infection. Therefore, ...

Journal: :The Journal of biological chemistry 1993
D L Jarvis M D Summers A Garcia D A Bohlmeyer

Foreign secretory pathway proteins are often produced in surprisingly low amounts in the baculovirus/insect cell expression system. One possible reason for this is that heterologous signal peptides might be inefficiently recognized by the insect cell protein translocation machinery. This idea was supported by a recent study showing that secretion of a plant protein in the baculovirus system was...

Journal: :Molecular biotechnology 2007
Kevin P O'Connell Elena Kovaleva James H Campbell Patricia E Anderson Susan G Brown David C Davis James J Valdes Richard W Welch William E Bentley Nikolai A van Beek

Infection of insect cells with baculovirus expression constructs is commonly used to produce recombinant proteins that require post-translational modifications for their activity, such as mammalian proteins. However, technical restraints limit the capacity of insect cell-based culture systems to be scaled up to produce the large amounts of recombinant protein required for human pharmaceuticals....

2013
Daniel Mendes Pereira Ardisson-Araújo Fabrício Da Silva Morgado Elisabeth Ferroni Schwartz Gerardo Corzo Bergmann Morais Ribeiro

Baculoviruses are the most studied insect viruses in the world and are used for biological control of agricultural and forest insect pests. They are also used as versatile vectors for expression of heterologous proteins. One of the major problems of their use as biopesticides is their slow speed to kill insects. Thus, to address this shortcoming, insect-specific neurotoxins from arachnids have ...

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