Step in procedure Supernatant haemoglobin (g) After thawing 0 45 First wash 1 6 Second wash 0-45 Third wash 0-12 Final product 0-023 After incubation 0-05 stability of the red cell preparation after incubation at 37°C for 30 minutes. Recovery was calculated from the supernatant haemoglobin in relation to the initial total haemo-globin after thawing (table 2). Mean (SD) recovery was 70% (13 3), ...

Nyka9) employed the Gomori-Grocott's chromic acid-methenamine silver method for demonstrating tubercle bacilli in tissue sections after sublimate fixative with added hydrogen peroxide or periodic acid. He described that this procedure could demonstrate mycobacteria that had lost their acidfastness by the usual carbol fuchsin stain. Harada5) also employed the periodic acid-methenamine silver sta...

Helicobacter pylori (Hp) gastritis is a worldwide problem significantly associated with duodenal and gastric peptic ulcer disease, gastric carcinomas, and MALT-type lymphomas. A simple, rapid, reproducible, reliable, and inexpensive stain to detect the organism in gastric biopsy specimens is thus of great value. To assess the reliability and cost-effectiveness of a novel Alcian yellow-toluidine...

In this paper, the optimal Fresnel lenses are designed by Zemax software.The fundamental problem of the Fresnel lenses is the beam divergence, which wasresolved by decreeing the output stain diameter on the image plane. For this purpose,two types of radial and cylindrical Fresnel lenses are simulated with different groovesusing Global and Hammer optimization methods. The minimum output stain di...

Negative stain electron microscopy (EM) allows relatively simple and quick observation of macromolecules and macromolecular complexes through the use of contrast enhancing stain reagent. Although limited in resolution to a maximum of ~18 - 20 Å, negative stain EM is useful for a variety of biological problems and also provides a rapid means of assessing samples for cryo-electron microscopy (cry...

When people understand noun-noun compounds such as kitchen mirror they generate a relationship between the two constituent nouns, combining them together in a new concept. How do people determine or construct the correct relational link between the constituents? In our first experiment, we present evidence against one approach to noun-noun compounds, namely that of specifying the meaning of a c...

An evaluation of the standardised Romanowsky stain of Marshall et al. has been made in a routine haematology laboratory. It was noted that this stain had several advantages over the May-Grünwald Giemsa stain used in most British laboratories. These advantages include ease and speed of preparation, a shorter staining time, and reproducibility of results. These results are described in detail. Th...

UTILITY: (1) Assess degree of fibrosis. H&E stain is not reliable for demonstration of fibrosis especially in early stages. Pericellular fibrosis around the central vein in steatohepatitis can be difficult to identify on the H&E stain. (2) Distinguish fibrosis from necrosis. In acute hepatitis, areas of confluent necrosis can be difficult to distinguish from fibrosis and can lead to an erroneou...