Rotaviruses (RVs) cause acute gastroenteritis in infants and young children, and are globally distributed. Within the infected host cell, RVs establish replication complexes in viroplasms ('viral factories') to which lipid droplet organelles are recruited. To further understand this recently discovered phenomenon, the lipidomes of RV-infected and uninfected MA104 cells were investigated. Cell l...

To identify previously unknown peroxisomal proteins, we established an optimized method for isolating highly purified peroxisomes from etiolated soybean cotyledons using Percoll density gradient centrifugation followed by iodixanol density gradient centrifugation. Proteins in highly purified peroxisomes were separated by two-dimensional PAGE. We performed peptide mass fingerprinting of proteins...

Percutaneous nephrolithotomy is indicated for renal stones larger than 2 cm and upper ureteral stones not amenable to retrograde management. Supracostal access may enable better access to the entire collecting system and even be preferential in some cases, although having a higher risk of pleural or lung injury, resulting in hydrothorax or pneumothorax. We report a case of a patient who develop...

LSEC Isolation Liver sinusoidal endothelial cells were isolated from wild type C567BL/6 mice by collagenase perfusion, iodixanol density gradient centrifugation and centrifugal elutriation, as described. Briefly, yields of LSEC are on average 80 million sinusoidal endothelial cells per 10 g liver with viability of more than 95% and purity greater than 96%, as determined by scanning electron mic...

Clinical observation shows that radiographic contrast media (CM) may influence thrombus formation. In the search for the underlying mechanism, we have shown that the ionic CM ioxaglate is a potent inhibitor of thrombin generation in platelet-poor and platelet-rich plasma, whereas the influence of the non-ionic contrast medium iodixanol is minimal. Ioxaglate boosts the inhibitory effect of the p...

One of the most promising gene transfer vectors in human clinical trials is AAV2. The quality of the vector preparations is a key element in obtaining reliable and reproducible data in preclinical studies. However, established protocols either result in impure, low infectious virus (CsCl2 gradient centrifugation) or demand a high level of manual and technical skills (CsCl2 gradient centrifugati...