We have developed an electrophoretic method to determine the size of a protein—DNA complex using the same binding and electrophoresis conditions as in an electrophoretic mobility shift assay (EMSA). The method is an adaptation of that of Ferguson (1), originally developed to determine the size of native proteins in non-denaturing gel systems. In SDS polyacrylamide gel electrophoresis, associati...

FROG skin has been used by many workers in the study of bioelectric phenomena of living membranes. The isolated living skin mounted with Ringer's solution on both sides maintains a potential of from 10 to 100 mV., the inside of the skin being positive. This potential can be modified by changing the chemical or physical conditions. Many workers have attempted to interpret the results of such cha...

BY THE USE OF TWO EXTREME MODELS: a hydrated sphere and an unhydrated rod the valence (net charge) of corpuscular proteins can be successfully calculated from electric mobility data by the Debye-Hückel theory (modified to include the effect of the ions in the ion atmosphere) in conjunction with the electrophoretic theory of Henry. As pointed out by Abramson, this permits a comparison with value...

The electrophoretic characteristics of the murine CL3 ascites tumour were investigated. Treatment of the cells with formaldehyde raised the electrophoretic mobility (E.P.M.) from — 1 06 to —1-28 /i/sec/V/cm; subsequent treatment with diazomethane reduced their mobility to zero. The E.P.M. of the diazomethane-treated cells did not alter over the pH range 3-0-8-0. This proved that the only ionic ...

the dependence on ionic strength of protonation of l-aspartic acid and its complexation withdioxouranium(vi) is reported in sodium perchlorate solution as a background salt. the measurementshave been performed at 25 ± 0.1 °c and various ionic strengths in the range 0.1 to 1.0 mol dm-3, using acombination of potentiometric and spectrophotometric techniques. the overall analysis of the present an...

In this work we have performed first-principles calculations based on the spin-polarized density-functional theory for the adsorption and diffusion of Au, Ag, and Pb atoms on NaCl 001 , KCl 001 , and KBr 001 surfaces. We consider also the influence of adatom charge on the adsorption and diffusion. In order to characterize the different systems we explicitly calculate charge transfer between sur...

Much of the molecular motion in the cytoplasm is diffusive, which possibly limits the tempo of processes. We studied the dependence of protein mobility on protein surface properties and ionic strength. We used surface-modified fluorescent proteins (FPs) and determined their translational diffusion coefficients (D) in the cytoplasm of Escherichia coli, Lactococcus lactis and Haloferax volcanii. ...