In the present study, we purified the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) which is involved in cellular energy production and has important housekeeping functions, from the ciliate Tetrahymena thermophila using a three-step procedure. The enzyme was purified ~68 folds by ammonium sulfate precipitation, followed by two steps of column chromatography (DEAE-cellulose...

Geoactive fungi play a significant role in bioweathering of rock and mineral substrates. Monazite is phosphate containing the rare earth elements (REE) cerium, lanthanum neodymium. Little known about geomicrobial transformations REE-bearing minerals which are also relevant to REE biorecovery from terrestrial extra-terrestrial reserves. The geoactive soil fungus Aspergillus niger colonized monaz...

The electrokinetic remediation of an agricultural soil contaminated with heavy metals was studied using organic acids as facilitating agents. unenhanced treatment deionized water processing fluid did not show any significant mobilization and removal due to the low solubilization precipitation at high pH conditions close cathode. EDTA citric acid 0.1 M were used agents favor dissolution transpor...

The two 4.6 kb chloramphenicol resistance (CmR) plasmids pSCS6 and pSCS7, previously identified in Staphylococcus aureus from subclinical bovine mastitis, both encoded an inducible chloramphenicol acetyltransferase (CAT, EC 2.3.1.28). The pSCS6- and pSCS7-encoded CAT variants were purified by ammonium sulphate precipitation, ion-exchange chromatography and fast protein liquid chromatography (FP...

An extracellular cytolytic toxin produced by the halophilic bacterium Vibrio vulnificus was isolated free of detectable contamination with medium constituents and other bacterial products by sequential ammonium sulfate precipitation, gel filtration with Sephadex G-75, hydrophobic interaction chromatography with phenyl-Sepharose CL-4B, and isoelectric focusing in an ethylene glycol density gradi...

BACKGROUND AND OBJECTIVE Artocarpus heterophyllus (jackfruit) is a latex producing plant. Plant latex is produced from secretory cells and contains many intergradients. It also has been used in folk medicine. This study aimed to purify and characterize the biological activities of a protease from jackfruit latex. MATERIALS AND METHODS A protease was isolated and purified from crude latex of a...

In recent years, there have been several papers dealing with the inhomogeneity of serum globulin and with the possibility of separating homogeneous components of constant and reproducible properties. Hewitt (7, 8) has reported that there are five different proteins, called euglobulin I and II, pseudoglobulin A, globoglycoid, and the “main pseudoglobulin fraction,” the latter not yet exactly def...

An extracellular, nonelastolytic, neutral metalloprotease of Serratia marcescens was purified by sequential ammonium sulfate precipitation, hydroxyapatite adsorption chromatography, flat-bed isoelectric focusing, and Sephadex G-100 gel filtration. The protease preparation had a 280/260 nm absorbance ratio of 1.8, was free of detectable amounts of endotoxin, carbohydrate, phosphorus, and other k...

proteomics has emerged since 1995 for studying the total proteome of cells, tissues, or organisms at any condition. the study of proteins has a fundamental importance because they are the functional and final molecules of the gene expression process and proteomics could be a valuable technique for achieving this purpose. mass spectrometry is a complementary technique for proteomics that allows ...

Although locusts can be sustainably produced and are nutrient rich, the thought of eating them can be hard to swallow for many consumers. This paper aims to investigate the nutritional composition of Locusta migratoria, including the properties of extracted locust protein, contributing to limited literature and product development opportunities for industry. Locusts sourced from Dunedin, New Ze...