The effects of KS-505a, a novel microbial metabolite, on the activity of calmodulin-dependent cyclic nucleotide phosphodiesterase (CaM-PDE) were investigated. (1) KS-505a potently inhibited the purified 61 kDa isoenzyme of CaM-PDE from bovine brain and required much higher doses to inhibit the purified 59 kDa isoenzyme of CaM-PDE from bovine heart. The inhibition of both isoenzymes was observed...

Fifty four patients with RA were included in the study. Blood samples were obtained from all cases and synovial fluid (SF) samples were obtained from 24 patients presenting with knee effusion. Besides routine tests of rheumatoid activity ESR, CRP, the following enzymes were analysed in serum and SF samples: alkaline phosphatase (ALP), LDH, 5’ nucleotidase (5’ NT) and adenosine deaminase (ADA), ...

in this study, we used random amplified polymorphic dna (rapd) for identification of 17 isolates of leishmania from the skin and reticuloendothelial system of humans, animal reservoirs (rodent and dog) and sandflies in various parts of iran in the last decade. fifteen species have been confirmed by isoenzyme characterization by the london school of hygiene and tropical medicine and shiraz unive...

Intracellular lactate dehydrogenase (LD) isoenzyme patterns were studied in the malignant cells of patients with a variety of lymphoid malignancies. These were compared with intracellular LD isoenzyme patterns of normal lymphoid cells and were also correlated with immunologic cell surface marker characteristics. Results showed that, in general, the malignant B cells of Burkitt's lymphoma and th...

A recent study found that an agarose gel electrophoresis (AGE) method yielded two distinct major bands corresponding to the hepatic and bone ALP isoenzymes (ALP2 ALP3, respec-tively) in bovine serum treated with protease neuraminidase (PN-treatment), although there were concerns intestinal isoenzyme (ALP5) often overlapped ALP3 human neuraminidase. Because ALP5 was separated from alone (P-treat...

treatment with PLA,. At this stage it is impossible to say whether the presence of such large amounts of lysophospholipids has a role in isoenzyme inactivation. Nevertheless it can be hypothesized that each P450 isoenzyme responds differently to changes in membrane NEFA content and composition. This situation might facilitate the selective modulation of P450 isoenzyme activity depending on the ...

We used an RIA and inhibition of enzyme activity to monitor the changes in mass and catalytic concentrations of the aspartate aminotransferase (EC 2.6.1.1;AST) isoenzymes in serum after myocardial infarction. Cytosolic (c-AST) and mitochondrial (m-AST) forms of AST were present in sera from all 38 of our patients. Although the immunological and catalytic concentrations of both isoenzymes correl...

Metastable creatine kinase MM isoenzyme was isolated and partially purified from homogenates of myocardium and skeletal muscle by gradient elution on carboxymethyl cellulose. This variant isoenzyme migrated between the MM and MB isoenzymes on agarose electrophoresis, accounted for 3.5% of the total creatine kinase activity in each tissue, was not a macromolecule, and had stable electrophoretic ...

Activity of serum/plasma beta-N-acetylhexosaminidase (EC 3.2.1.52) was determined by means of a continuous spectrophotometric method using 3,3'-dichlorophenylsulphonphthaleinyl-N-acetyl-beta-D-glucosaminid e as substrate, with very satisfactory results. Incubation of an undiluted aliquot (1 ml) of samples at 52 degrees C for 8 hours with an adjusted pH 5.5-6.0 provoked only the inactivation of ...