Accurate label-free methods or assays to obtain the initial reaction rates have significant importance in fundamental studies of enzymes and in application-oriented high throughput screening of enzyme activity. Here we introduce a label-free approach for obtaining initial rates of enzyme activity from heat measurements, which we name initial rate calorimetry (IrCal). This approach is based on o...

Adsorption microcalorimetry measures the energetics of adsorbate-surface interactions and can be performed by use of several different techniques. This review focuses on three methods: single-crystal adsorption calorimetry (SCAC), isothermal titration calorimetry (ITC), and electrochemical adsorption calorimetry. SCAC is a uniquely powerful technique that has been applied to a variety of atoms ...

Ion exchange chromatography is a widely used method for purification of all types biomolecules in current biotechnological downstream processes. Knowledge on the binding behavior proteins provides valuable insight understanding molecular mechanisms protein interactions biological context. However, thermodynamic parameters such as enthalpy and entropy changes that characterize adsorption are sti...

The above article, first published online as an Accepted Article on 23 September 2021 in Wiley Online Library (wileyonlinelibrary.com), has been withdrawn by agreement between the authors, Editor-in-Chief and Wiley-VCH Verlag GmbH. Due to a technical issue article was erroneously twice.