The aim of this study was to evaluate the efficacy of a hyaluronic acid containing eye drop in ameliorating ocular surface pathology and discomfort in canine keratoconjunctivitis sicca (KCS). Twenty five dogs with KCS treated with a topical carbomer (CA)-based tear replacement gel were moved to treatment with a hyaluronic acid (HA)-containing tear replacement eye drop. Dogs were subject to a fu...

It has been widely postulated that the central mechanism of hepatic reperfusion injury involves the conversion, during ischemia, of the enzyme xanthine dehydrogenase (XDH) to its free radical-producing form, xanthine oxidase (XOD). However, this theory has been questioned because (a) XDH to XOD conversion in whole liver occurs very slowly; (b) the cellular distribution of XDH/XOD is unclear; an...

Retinoids influence growth and differentiation of keratinocytes (KCs) and are widely used for the management of skin diseases and for prevention of nonmelanoma skin cancer (NMSC) in predisposed patients. Here we investigated the effect of all-trans-retinoic acid (ATRA) on KC apoptosis. When KCs were cultured in confluent monolayers for several days, they acquired resistance against UVB-induced ...

Hepatic ischemia/reperfusion (I/R) contributes to major complications in clinical practice affecting perioperative morbidity and mortality. Recent evidence suggests the key role of nucleotide-binding oligomerization domain-like receptor (NLR) family pyrin domain-containing 3 (NLRP3) inflammaosme activation on the pathogenesis of I/R injury. Asiatic acid (AA) is a pentacyclic triterpene derivati...

Slac2-b, also known as exophilin-5, is a Rab27b effector protein with role in exosome transport and encoded by the EXPH5 gene. We previously described biallelic loss-of-function mutations an autosomal recessive form of epidermolysis bullosa simplex. However, how loss Slac2-b expression leads to skin fragility erosions unknown. In this study, we demonstrate that keratinocytes (KCs) isolat...

This paper presents a novel approach for optimal key characteristics-based sensor distribution in a multistation assembly process, for the purpose of diagnosing variation sources responsible for product quality defects in a timely manner.. Current approaches for sensor distribution are based on the assumption that measurement points can be allocated at arbitrary locations on the part or subasse...

Aberrant STING and Interferon (IFN) signaling are hallmarks of SLE, a female-biased disease, where amplified type I IFN responses by keratinocytes (KCs) lead to increased Stimulated Genes (ISGs) expression. Here we show that HERC6, which encodes for an E3 ubiquitin ligase, with unknown role in the skin, is induced basal layer human SLE epidermis coinciding marked decrease number sex-biased gene...

Prurigo nodularis (PN) is a chronic, inflammatory skin condition characterized by intensely pruritic, hyperkeratotic nodules that disproportionately affects Black patients. Little known about the interaction of innate immune cells with epithelial and stromal elements in PN. Thus, we utilize imaging mass cytometry (IMC) to examine cutaneous lymphocytic myeloid cell populations lesional PN skin, ...