نتایج جستجو برای: keywordschicken sybr green rt

تعداد نتایج: 199695  

2012
Qian Mei Xiang Li Yuanguang Meng Zhiqiang Wu Mingzhou Guo Yali Zhao Xiaobing Fu Weidong Han

BACKGROUND The spatiotemporal expression patterns of microRNAs (miRNAs) are important to the verification of their predicted function. RT-qPCR is the accepted technique for the quantification of miRNA expression; however, stem-loop RT-PCR and poly(T)-adapter assay, the two most frequently used methods, are not very convenient in practice and have poor specificity, respectively. RESULTS We hav...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تربیت مدرس - دانشکده علوم پزشکی 1387

چکیده ندارد.

Journal: :International Journal of Molecular Sciences 2008
Christoph Brandfass Petr Karlovsky

Fusarium graminearum Schwabe (Gibberella zeae Schwein. Petch.) and F. culmorum W.G. Smith are major mycotoxin producers in small-grain cereals afflicted with Fusarium head blight (FHB). Real-time PCR (qPCR) is the method of choice for species-specific, quantitative estimation of fungal biomass in plant tissue. We demonstrated that increasing the amount of plant material used for DNA extraction ...

2016
Brigitte Bruijns Roald Tiggelaar Han Gardeniers

In this research six cyanine fluorophores for the quantification of dsDNA in the pg-ng range, without amplification, are compared under exactly identical conditions: EvaGreen, SYBR Green, PicoGreen, AccuClear, AccuBlue NextGen and YOYO-1. The fluorescence intensity as a function of the amount of dsDNA is measured at the optimal wavelengths for excitation and emission and for each dye the limit ...

Journal: :Journal of clinical microbiology 2002
Carmen Aldea Carmen P Alvarez Lola Folgueira Rafael Delgado Joaquín R Otero

We have evaluated a real-time PCR procedure based on the LightCycler technology for rapid detection of herpes simplex virus (HSV) in genital lesions. Two sets of primers, corresponding to the thymidine kinase and DNA polymerase regions, were used for the amplification reactions in separate capillaries containing the SYBR Green I dye as detection signal. In 28 of 118 samples (24%), HSV was isola...

Journal: :Microbiological research 2006
F Fernández J Gutiérrez A Sorlózano J M Romero M J Soto F Ruiz-Cabello

A comparative study was conducted of a novel real-time quantitative PCR test (LightCycler System) with FastStart DNA Master(PLUS) SYBR Green I dye to detect DNA of human herpes virus 6 (HHV-6). Results were compared with those of a real-time quantitative PCR with hybridization probe (HP) formats using the fluorescence resonance energy transfer method, and with those of a single qualitative PCR ...

Journal: :Applied and environmental microbiology 1998
M G Weinbauer C Beckmann M G Höfle

High background fluorescence and unspecific staining hampered the epifluorescence enumeration of bacteria in 45% of the tested soil and sediment samples with 4',6-diamidino-2-phenylindole (DAPI) and polycarbonate membrane filters. These problems of the determination of total cell counts can be circumvented by using green fluorescent high-affinity nucleic acid dyes and aluminum oxide membrane fi...

Journal: :Journal of clinical microbiology 2006
M M Parida S R Santhosh P K Dash N K Tripathi P Saxena S Ambuj A K Sahni P V Lakshmana Rao Kouichi Morita

The standardization and validation of a one-step, single-tube accelerated quantitative reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay is reported for rapid and real-time detection of Japanese encephalitis virus (JEV). The RT-LAMP assay reported in this study is very simple and rapid; the amplification can be obtained in 30 min under isothermal conditions at 63 degr...

Journal: :Journal of clinical microbiology 2004
Xiaoli Pang Bonita Lee Linda Chui Jutta K Preiksaitis Stephan S Monroe

We developed an assay for the detection and quantitation of norovirus with the LightCycler SYBR Green-based real-time reverse transcription-PCR (real-time LC RT-PCR) and previously published primers in the capsid and the polymerase gene. One hundred thirty-two stool specimens from the Provincial Laboratory for Public Health (Microbiology), Alberta, Canada, and the Centers for Disease Control an...

Journal: :jundishapur journal of microbiology 0
mohammad soleimani department of microbiology, qom branch, islamic azad university, qom, ir iran; department of microbiology, qom branch, islamic azad university, qom, ir iran, p.o. box: 37185/364. tel:+98-2517780001, fax: +98-2517780001 mohammad reza zolfaghari department of microbiology, qom branch, islamic azad university, qom, ir iran abbas morovvati department of microbiology, qom branch, islamic azad university, qom, ir iran

background aggregatibacter actinomycetemcomitans and tannerella forsythensis are two major pathogens in destructive periodontal disease in humans. the detection of these bacteria is needed for diagnosis and management of the mentioned diseases. objectives we aimed to develop and compare improved multiplex conventional and sybr green real time pcr assays for a specific diagnosis of the organisms...

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