Current methods to study angiogenesis in cancer growth and development can be difficult and costly, requiring extensive use of in vivo methodologies. Here, we utilized an in vitro adipocyte derived stem cell and endothelial colony forming cell (ADSC/ECFC) co-culture system to investigate the effect of lentiviral-driven shRNA knockdown of target genes compared to a non-targeting shRNA control on...

Ribonucleotide reductase (RR) has an essential role in DNA synthesis and repair and is a therapeutic target in a number of different cancers. Previous studies have shown that RNAi-mediated knockdown of either the RRM1 or RRM2 subunit sensitizes cells to the cytotoxic effects of the nucleoside analogs and more recently it has been shown that RRM2 knockdown itself has a growth inhibitory effect. ...

The poultry mite, Dermanyssus gallinae, is a parasite of fowl and birds worldwide, causing huge economic losses in production systems. We investigated gene-knockdown approach in identifying potential vaccine antigens, targeting Histamine Releasing Factor (HRF) and Cathepsin D (C), through immersion in dsRNA derived from the two genes, for 7 and 16 h respectively, alongside LacZ control. Quantit...

USING GENE KNOCKOUT STRATEGIES to help define the physiological and/or pathophysiological role(s) of factors produced by specific renal cell types presents substantial challenges. Renal cell-specific gene targeting, involving a Cre recombinase (Cre)-expressing transgene (either constitutively expressed or inducible) and a loxP-flanked (floxed) target allele, has been achieved in vivo in mouse p...

Antisense oligonucleotides (ASOs) are synthetic, single-strand RNA-DNA hybrids that induce catalytic degradation of complementary cellular RNAs via RNase H. ASOs are widely used as gene knockdown reagents in tissue culture and in Xenopus and mouse model systems. To test their effectiveness in zebrafish, we targeted 20 developmental genes and compared the morphological changes with mutant and mo...

Production of knockout mice using targeted embryonic stem cells (ESCs) is a powerful approach for investigating the function of specific genes in vivo. Although the protocol for gene targeting via homologous recombination (HR) in ESCs is already well established, the targeting efficiency varies at different target loci and is sometimes too low. It is known that knockdown of the Bloom syndrome g...

Background Cell motility and migration are known to be regulated by the Rho family of GTPases through their effects on the actin cytoskeleton. In breast cancer studies, RhoC has been identified as a highly specific marker in detecting tumors that developed metastases. This study aims to investigate the impact of targeting RhoC in human breast cancer cells by utilising ribozyme transgene technol...

Aberrant expression of B-tubulin isotypes is frequently described in tumor tissues and tubulin-binding agent (TBA)–resistant cell lines. There is limited understanding of the role of specific B-tubulin isotypes in cellular sensitivity to TBAs, and to gain insights into the functional role of BIIand BIVb-tubulin, we examined these isotypes in lung cancer cell lines NCI-H460 (H460) and Calu-6. Dr...

conclusions the proliferation and invasion of human bladder cancer cells can be inhibited by rnai-targeting skp2. as a result, skp2 may be a potential target for gene therapy in cases of human bladder cancer. materials and methods the expression of the skp2 gene was knocked down by rna interference (rnai) in t24 cells. the transcription level of skp2 was detected by reverse transcription-quanti...

In this study we investigated the correlation between RhoC expression and cancer stem cells (CSCs) formation in head and neck squamous cell carcinoma (HNSCC). The inhibition of RhoC function was achieved using shRNA. The expression of stem cell surface markers, ALDH and CD44 were significantly low in two RhoC depleted HNSCC cell carcinoma cell lines. Furthermore, a striking reduction in tumorsp...