BACKGROUND The relation between elevated blood lactate level and mortality and morbidity rates after coronary bypass surgery is a proven subject. One of the factors that seems to affect directly the blood lactate level is the storage duration of packed red blood cells. OBJECTIVES This study aimed to evaluate the effect of storage duration of transfused blood on serum lactate during cardiac su...

Malate Dehydrogenase-The enzyme was prepared from B. subtilis and recrystallized at least three times (4). The crystalline enzyme was stored at 5” in partially saturated (about 50%) ammonium sulfate containing 0.01 M NazHP04-KH,P04 (pH 7.7) and 0.001 M EDTA. Chemicals-Amino acids, ketocarboxylic acid, and hydroxycarboxylic acid were of the highest grade commercially available. They were purchas...

1. Acetoacetate or short-chain fatty acids (acetate, butyrate, propionate, n-hexanoate, n-octanoate) accelerate the rate of glucose formation from lactate, fumarate and other precursors in slices of kidney cortex (rat, rabbit, sheep). The cause of this acceleration has been investigated. 2. There are two different mechanisms of acceleration. At low concentrations of glucogenic precursors the ac...

The metabolic effects of epinephrine on the carbohydrate metabolism of muscle tissues have been extensively studied both in vivo and in vitro, but conflicting evidence makes it uncertain whether decreased glucose utilization by muscle contributes significantly to the hyperglycemia produced by this hormone (l-4). An increased conversion of phosphorylase b to a and increased glycogenolysis are wi...

The developmental changes in glutamine synthetase activity in rat liver and brain have been studied and shown to be different in the two tissues. The liver enzyme exhibited a fall in activity after birth, followed by a slow recovery and a rapid increase in activity for a short interval of time, before a gradual rise to the adult level in about 40 days. The brain enzyme raised its activity gradu...

Thank you, Ben. I appreciate the opportunity to speak today and be part of this symposium. I do not have any relationships, financial or other conflicts of interest related to this topic, however, I do want to disclose that I am the default speaker or “weakest link” on this program today. Six months ago, when Ben and I started to talk about this session, we had a “dream team” of speakers in min...

Ljungdahl, Lars (Western Reserve University, Cleveland, Ohio), and Harland G. Wood. Incorporation of C(14) from carbon dioxide into sugar phosphates, carboxylic acids, and amino acids by Clostridium thermoaceticum. J. Bacteriol. 89:1055-1064. 1965.-The mechanism of synthesis of acetate from carbon dioxide by Clostridium thermoaceticum was investigated by incubating cells with glucose or xylose ...

BLANCHAER, M. C. Respiration of mitochondria of red and white skeletal muscle. Am. J. Physiol. 206(5) :1o15-1020. I g64.-Mitochondria of guinea pig red and white skeletal muscle were isolated in a medium containing 0.25 M sucrose, I mM ethylenediamine tetraacetate, and I y0 fraction V bovine albumin. Oxidative phosphorylation with pyruvate-malate as substrate was tightly coupled in both types o...

Inhibition of fermentation or of glucose uptake in the presence ofoxygen has been observed in many forms of life and is generally referred to as the 'Pasteur effect'. In most tissues, oxidative inhibition of fermentation (ethanol or lactate production) is reflected by a parallel decrease in glucose consumption. Since certain tissues utilize lactate significantly, estimation of the Pasteur effec...

Dimitri Renard, MD Helene Bonnaure, MD Pierre Labauge, MD, PhD A 46-year-old woman with a history of seizures, migraine, diabetes mellitus, and neurosensory hearing loss presented with progressive gait unsteadiness. Her mother had the same clinical features. Examination revealed short stature, cognitive deficit, and ataxia. Brain MRI showed diffuse atrophy and posterior leukoencephalopathy (fig...