Rheumatoid factors (RF) are autoantibodies with specificity for the Fc portion of human IgG. Production of RF is a characteristic feature of rheumatoid arthritis (RA) and is detectable in high titer in about 90% of these patients. In this study, we measured total IgM, IgA, IgMRF and IgARF in serum and synovial fluid of 45 RA patients by ELISA and the results were compared with those obtained b...

Background: Rotavirus is a major cause of acute diarrhea in children worldwide. It is an important cause of death among young children in developing countries. The aim of this study was to evaluate the prevalence of rotavirus infection in children less than five years old hospitalized for gastroenteritis in Jahrom. Methods: One hundred and two stool samples were collected from children less tha...

Fatal toxoplasmosis was diagnosed in a Blanford's fox (Vulpes cana) from the United Arab Emirates. Toxoplasma gondii-like tachyzoites were found associated with necrosis in intestine, spleen, liver, kidneys, lungs, skeletal muscle, brain and heart. Protozoal tachyzoites reacted positively with T. gondii-specific polyclonal antibodies. Antibodies to T. gondii were detected in 10 of 12 V. cana as...

C-Reactive protein (CRP) is an acute-phase protein that increases during systemic inflammation and is currently one of the most frequently studied inflammatory markers in epidemiology. We have determined CRP concentration using novel latex reagent with polyclonal antibody. In the present study, we determined the concentration of CRP using monoclonal antibodies, and evaluated the interaction of ...

In this study, sLPS antigen coated latex bead was prepared and their evaluation was done along with RBPT, commonly employed for the serodiagnosis of brucellosis. sLPS was extracted from Brucella abortus S99 and coated on polystyrene latex beads in a phosphate buffer saline coating buffer. A total of 200 samples were collected from unorganized dairy farm and tested with RBPT and sLPS based latex...

Methicillin (oxacillin)-resistant staphylococci (MRS) have emerged as major clinical and epidemiological pathogens, and there have been frequent reports of MRS infections in the veterinary field. The MRSA-Screen latex agglutination test (Denka Seiken Co., Ltd., Tokyo, Japan) was compared with an oxacillin agar screen test, MIC determination, and mecA PCR assay, the "gold standard." In an analys...

The authors evaluated a simple latex agglutination test for the serodiagnosis of acute human leptospirosis. A total of 380 serum samples from 85 confirmed leptospirosis patients and 202 non-leptospirosis patients were selected Using the selected cut-off value of weakly positive, the overall sensitivity and overall specificity of the test were 94.1% and 97.0% respectively. The weighted kappa val...

Burkholderia pseudomallei is the causative agent of melioidosis. In northeast Thailand, this gram-negative bacterium is a major cause of mortality from septicemia. The definitive diagnosis of this disease is made by bacterial culture. In this study, we produced a monoclonal antibody (MAb) specific to the 30-kDa protein of B. pseudomallei by in vivo and in vitro immunization of BALB/c mice with ...

Although latex agglutination assays have been used for some years to diagnose thrombotic disorders, only recently has it been possible to measure specifically the products of fibrin breakdown in the presence of fibrinogen degradation products, by using monoclonal antibodies. We have evaluated a preparation of latex particles coupled to the monoclonal antibody DD-3B6/22, which is specific for cr...

The latex-fixation test (Singer and Plotz, 1956) is widely used as a serological criterion for rheumatoid arthritis (Alexander and McCarthy, 1966; Walton, 1968). The test is often carried out in serial dilution, by which a titre as well as an agglutination pattern is obtained. The usual practice is to choose a borderline titre in an empirical way and to call all titres above the borderline valu...