The objective of this study was to determine the toxicity of the neem biopesticide azadirachtin (NEEM-ON, Brand name) on the freshwater fish ‘Labeo rohita’. Fishes were exposed to various concentrations of botanical insecticide azadirachtin for 96 h and the percent mortality was recorded. The 96h LC50 value determined by Finney’s Probit Analysis Method was found to be 42.66 ppm. Behavioural pat...

Epilachna vigintioctopunctata is an important pest that causes considerable economic losses to many crops including egg-plants. The crude aqueous extracts of leaves from three indigenous plants namely Ricinus communis, Calotropis procera and Datura metel were used against this beetle aiming at its control under laboratory conditions. Larvicidal bioassays of the extracts showed the following ord...

The acute toxicity of ammonia on Thulinius ruffoi (Bertolani, 1981), a eutardigrade isolated from a small waste water treatment plant (WWTP) in Poland, was estimated. Our results show that no active individuals survived a 24 h exposure to solutions equal to or higher than 125 mg/L of total ammonia nitrogen (NH3-N + NH4 (+)-N), which, under the conditions in our experiment, was equivalent to 1.1...

BACKGROUND Protecting the environment from chemical hazards of synthetic insecticides along with offering of new breeding areas for vectors by urbanization indicate the trial of natural insecticides. METHODS The acetone extracts of Anethum graveolens, Ocimum basilicum and Thymus vulgaris were tested for their insecticidal effect on Culex pipiens adults and larvae in different concentrations d...

The aim of this study was to select different genotypes of Lippia sidoides with the highest activity against larvae and engorged females of the tick Rhipicephalus (Boophilus) microplus. The germplasms studied were LSID006, LSID102, LSID103 and LSID104. The LSID104 genotype, that presented carvacrol as a major constituent, was the germplasm with the worst larvicide effect (LC50 2.99 mg/mL). The ...

BACKGROUND The leaves and roots of Cissampelos mucronata A. Rich (Menispermaceae) are widely used in the tropics and subtropics to manage various ailments such as gastro-intestinal complaints, menstrual problems, venereal diseases and malaria. In the Coast region, Tanzania, roots are used to treat wounds due to extraction of jigger. Leaves of Tephrosia villosa (L) Pers (Leguminosae) are reporte...

The crude methanolic extract of Averrhoa bilimbi Linn. (Oxalidiaceae) fruits and its different fractions have been investigated for the evaluation of in vitro cytotoxic potential. The dried and powder fruits were extracted with methanol at room temperature and the concentrated methanolic extract was fractionated by the modified Kupchan partitioning method to provide pet-ether, carbon tetrachlor...

Using twenty chemicals commercially available or provided by NCI, we demonstrated the feasibility of using zebrafish embryos to study toxicity response by assessing Median Lethal Concentration (LC50), developmental teratogenicity, and specific liver and kidney toxicity. For the twenty compounds used to study LC50 and liver and kidney toxicity in zebrafish embryos, we found good correlation with...

background: pesticides are widely used in agriculture. excessive use of pesticides has health risk for human and threatens non-target organisms. this research aimed to determine lethal concentrations of malathion and hinosan for carassius auratus (5±1 gr) [mean ± sd]. methods: experiments were performed according to o.e.c.d for 4 days (96 h) and concentrations of 0, 1, 2, 4, 8 mg l-1 hinosan an...

Arsenic (As) is a human carcinogen. Our prior work showed that chronic (>18 weeks) low level (500 nM) arsenite (As3+) exposure induced malignant transformation in a rat liver epithelial cell line (TRL 1215). In these cells, metallothionein (MT) is hyper-expressible, a trait often linked to metal tolerance. Thus, this study examined whether the adverse effects of arsenicals and other metals were...