Rye is an important crop in Northern and Eastern Europe. However, the application of various biotechnologies in rye breeding has been limited duo to its recalcitrant in tissue culture. In order to improve somatic tissue effi ciency, key factors affecting somatic embryogenesis and reproducible green plant regeneration of rye (Secale cereale L.) were evaluated and optimised. In this study, a tota...

Ficus lyrata is a popular ornamental foliage plant with unique violin- or guitar-shaped green leaves. In our previous study, grapevine gene VvMybA1 was introduced into F. via Agrobacterium-mediated transformation, which resulted in the availability of purple-leaved plants. Since transcription factor, regeneration transgenic plants might potentially produce variants multicolored The objective th...

<i>Verbascum scamandri</i> Murb. known as "Kazdağı Mullein" is an endemic species in Türkiye and classified endangered (EN) species. The aim of this study to establish efficient callus culture for <i>V. analyze the amounts verbascoside, luteolin, aucubin metabolites calli samples. Leaf explants were cultured on MS medium with cytokinin (BAP, Kin, 0, 0.5, 1, 2, 3 mg/L) auxin (N...

It has been observed in this laboratory (15) that the normally polar distribution of callus growth on tobacco stem explants becomes non-po'ar in the presence of low levels of 2,3,5-triiodobenzoic acid (TIBA), while the amount of callus growth is not diminished. It was thought that this phenomenon might reflect a specific inhibition by TIBA of the polar transport of auxin. Subsequent studies (10...

The midto long-term preservation of papaya (Carica papaya L.) would allow for the safeguarding of important germplasm. In this study, soft friable callus (SFC) and hard callus (HC) were induced from the first two true leaves of 10-day-old seedlings containing a midrib derived from the germinated seed of two cultivars (‘Rainbow’ and ‘Sunrise Solo’). Following germination on a Murashige and Skoog...

Some specific microorganisms have been identified as etiologic agents of periodontitis. The main goal of treatment is to control the spread of infection. Depending on the extent of the gum disease, treatment varies. Therapeutic antibiotic along with surgery are the most opted form of treatment. But, due to high costs of antibiotics, the disastrous side effects and emergence of multi drug resist...

Crassocephalum rubens is a wild, economic and medicinal plant that is now threatened by genetic erosion in Nigerian ecosystem. The scarcity of the plant is due to indiscriminate collection from the wild and the difficulty in germinating the seeds. This study provides protocols for in-vitro propagation of C. rubens to supplement natural propagation, contribute to conserve the plant genetic resou...

The first experiment was done to select the most suitable explant establish a culture for shoot regeneration. Four different explants (cotyledon, cotyledon node, leaf and hypocotyl) excised from in vitro grown seedlings were cultured on MS medium containing 1.0 mg l-1 BAP 0.2 NAA. Cotyledon responsive which exhibited quick callus initiation. second conducted determine effect of media BAP, NAA a...

Iron (Fe) deficiency induces chlorosis (IDC) in plants and can result in reduced plant productivity. Therefore, development of Fe-efficient plants is of great interest. To gain a better understanding of the physiology of Fe-efficient plants, putative novel plant variants were regenerated from potato (Solanum tubersosum L. var. 'Iwa') callus cultures selected under Fe deficient or low Fe supply ...

This paper describes a protocol for the efficient vegetative propagation of Cleome rosea by somatic embryogenesis. Leaf and stem explants from nursery-grown seedlings of C. rosea were cultivated on Murashige and Skoog (MS) medium supplemented with indole-3-acetic acid (IAA), α-naphthaleneacetic acid (NAA), 4-amino-3,5,6trichloropicolinic acid (picloram) or 2,4-dichlorophenoxyacetic acid (2,4-D)...