The glycoside hydrolase LXYL-P1-2 from Lentinula edodes can specifically hydrolyze 7-β-xylosyltaxanes to form 7-β-hydroxyltaxanes for the semi-synthesis of paclitaxel. In order to improve the catalytic properties of the enzyme, we performed error-prone PCR to construct the random mutant library of LXYL-P1-2 and used the methanol-induced plate method to screen the mutants with improved catalytic...

To obtain rich in organic forms of zinc extracts of Lentinus edodes, possessing putative higher immunostimulating properties than actually used, mycelia were cultivated in zinc(II) enriched media. Culture media were enriched in zinc in concentration ranging from 0 to 90 microg mL(-1), added to the medium before inoculation. Total zinc concentration in submerged cultivated mycelial biomass has b...

In order to isolate the genes expressed specifically and abundantly in the mature fruit body of Lentinula edodes, the cDNAs derived from the gill of the fruit body were compared with the cDNAs from the mycelia by differential screening. Consequently, six clones were identified as fruit-body-specific genes (fbg03, 08, 13, 14, 16, and 21). The deduced amino acid sequence of fbg14 (Le.cypfb) had s...