Dietary introduction of bacterially expressed double-stranded RNA (dsRNA) has great potential for management of Leptinotarsa decemlineata. Identification of the most attractive candidate genes for RNA interference (RNAi) is the first step. In the present paper, three complete chitin synthase cDNA sequences (LdChSAa, LdChSAb and LdChSB) were cloned. LdChSAa and LdChSAb, two splicing variants of ...

RNA interference (RNAi) is a naturally occurring phenomenon in eukaryotes in which a double-stranded RNA (dsRNA) suppresses the expression of a target gene. RNAi has markedly changed the way in which functional genetics studies are performed, especially in non-model organisms. In insects, the efficacy of RNAi is influenced by several factors, including the species and the methods of dsRNAs deli...

1 The attractiveness of potato plants treated with a synthetic host volatile blend [(Z)-3-hexenyl acetate (þ/–)-linalool, and methyl salicylate] to newly emerged and 5-day-old adult Colorado potato beetle, Leptinotarsa decemlineata (Say), was compared at four doses against untreated control plants and plants treated with an azadirachtin-based antifeedant in greenhouse cage arenas. 2 Attractant-...

Keys in Spanish and English are given for the genera of Chrysomelinae known from Costa Rica. For each genus, a list of species compiled from collections in the University of Costa Rica, the National Biodiversity Institute, and the entomological literature is presented. The genus Planagetes Chevrolat 1843 is recorded for the first time from Central America, and the genus Leptinotarsa Stål 1858 i...

The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most damaging insect pests of potato, Solanum tuberosum L. Novaluron is a relatively new benzoylphenyl urea insect growth regulator with good activity against this pest. Earlier studies revealed that feeding on potato foliage treated with novaluron induces reversible egg hatch inhibition in adult Colorado potato beetles....