A modified TaqMan real-time polymerase chain reaction targeting a 138bp fragment within the lipl32 gene was developed to identify exclusively pathogenic Leptospira spp. in dog urine samples. Thirty-five samples from dogs with suspected clinical leptospirosis and 116 samples from apparently healthy dogs were tested for presence of leptospiral DNA using the TaqMan-based assay. The results were co...

OBJECTIVE Determining Leptospira spp . antibody seroprevalence in an apparently healthy population and in groups at risk plus their associated factors in the town of Villavicencio in the Meta department of Colombia MATERIALS AND METHODS A cross-sectional epidemiological model was used (subjects being selected by convenience sampling). Blood-samples were obtained by cubital puncture from (n: 5...

Leptospirosis is caused by spirochete bacteria of the genus Leptospira and considered most widespread zoonosis worldwide. It an important agent that causes animal production to decrease. In cattle, it affects especially reproductive tract. The objective this study was determine seroprevalence spp., molecularly detect in tissues aborted fetuses, identify main risk factors associated with infecti...