Telomere length is a potential biomarker of aging and risk for age-related diseases. For measurement of relative telomere repeat mass (TRM), qPCR is typically used primarily due to its low cost and low DNA input. But the position of the sample on a plate often impacts the qPCR-based TRM measurement. Recently we developed a novel, probe-based Luminex assay for TRM that requires ~50ng DNA and inv...

The aim of this study was to analyse the correlation between a new multiplex qPCR assay and a reference qPCR assay for assessment of the human papillomavirus (HPV16) load and the viral genome status. The study was performed on 100 HPV16 positive samples containing premalignant lesions and carcinomas. HPV16 E2 and E6 gene loads were assessed by two PCR methods. The load of E2 and E6 was normaliz...

Scientific conferences fulfil many roles, but one of the most mportant ones is that they help shape the direction in which a scintific discipline grows by promoting person-to-person exchanges f information, ideas and constructive criticisms between scientists rom different backgrounds. This interaction also helps to idenify areas of controversy and promotes efforts to address and, it is oped, r...

Since the invention of real-time PCR (qPCR), thousands of high-impact studies have been conducted and published using qPCR technique (Heid et al. 1996; Higuchi et al. 1993; VanGuilder, Vrana, and Freeman 2008). Because it is highly sensitive, qPCR is the preferred method for microarray data validation (Canales et al. 2006); however the most exciting applications have been in the discovery of ne...

Background Patients benefiting from the advent of JAK inhibitors has increased in treatment rheumatoid arthritis(RA). However, it is also known that there are RA cases do not respond sufficiently to inhibitors(JAKi). In order increase number refractory arthritis cases, necessary establish following methods for JAKi-resistant cases. Objectives We analyzed efficacy bDMARD switcher group and JAKi ...

Anabaena planktonica is a harmful, bloom-forming freshwater cyanobacterium, which has arrived recently in New Zealand. In the short time since its incursion (<10 yr), A. planktonica has spread rapidly throughout lakes in the North Island. To date, the identification and enumeration of A. planktonica has been undertaken using light microscopy. There is an urgent demand for a highly sensitive and...

background: type 1 diabetes (t1d) is caused by cell-mediated autoimmune attack on pancreatic beta-cells. previous studies highlight the role of micrornas (mirnas) in the pathogenesis of t1d. mirnas are small non-coding rnas involved in the regulation of gene expression post-transcriptionally. in this work, mir-18b was chosen and the differential expression of it was measured between t1d patient...