Retroviral vectors with long terminal repeats (LTRs), which contain strong enhancer/promoter sequences at both ends of their genome, are widely used for stable gene transfer into hematopoietic cells. However, recent clinical data and mouse models point to insertional activation of cellular proto-oncogenes as a dose-limiting side effect of retroviral gene delivery that potentially induces leukem...

OBJECTIVE To investigate the effect of all-trans retinoic acid(ATRA) on the proliferation and differentiation of brain tumor stem cells(BTSCs) in vitro. METHODS Limiting dilution and clonogenic assay were used to isolate and screen BTSCs from the fresh specimen of human brain glioblastoma. The obtained BTSCs, which were cultured in serum-free medium, were classified into four groups in accord...

  Egg drop syndrome (EDS) is caused by a hemagglutinating adenovirus which has become a major cause of lost egg production and sever economic losses in breeder and layer chicken flocks throughout the world. A PCR assay was optimized for detection of EDS virus in inoculated allantoic fluids (AFs) of duck and chicken emberyonated eggs. Two strains of EDS viruses were propagated in allantoic cavit...

exploration of natural products with antitumoral effects such as etoposide, tenioposide, camptothecin and paclitaxel from conifers provoked us to do some research on anti-tumoral activity of iranian medicinal plants. ten different species of iranian conifers aerial parts were collected across the country. the relative potency of methanolic extract were investigated on the inhibition of topoisom...

Abstract The aim of the present study was to investigate effects milk composition changes on in vitro growth bovine mastitis pathogens. Nutritional requirements three major pathogens Escherichia coli ( E. ), Staphylococcus aureus S. and Streptococcus uberis ) were investigated . We used ultra-high temperature (UHT) treated with different contents fat, protein, carbohydrates test influence avail...

As the limiting substrate is altered, a microorganism’s internal structure is also altered by invoking different metabolic pathways for the utilization of the limiting substrate. Transitions between pathways are moderated by the processes of metabolic regulation and are observed under steady-state and transient conditions. Under nitrogen-limited steady-state conditions, the effects of overflow ...