Dispersive liquid-liquid microextraction method (DLLME) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to determine thiamine (B1), nicotinamide (B3) and pyridoxine (B6) in sour cherry juice. This method was rapid, simple and sensitive. Separation was accomplished using a C18 column. The optimum chromatographic conditions were found to be: mobile ph...

Step 1 Extraction One of two methods can be used. In solid phase extraction the sample is put on a plate coated with a substance that organic compounds (such as drugs) do not adhere to, but to which other compounds in the sample do, and then the organics washed off with a suitable solvent. In liquid-liquid extraction an organic solvent is mixed with the sample, the organic compounds migrate to ...

The report deals with liquid-liquid distribution systems, in which one phase is aqueous, and the distribution of the component can be influenced in many cases with chemical reactions. The formulas and equations are given, by which the actual distribution ratio of the analyte at given conditions and concentrations can be predicted. The conditional extraction constants, which are essential for th...

Background and Objectives: A method was developed and validated for determining 15 polycyclic aromatic hydrocarbons (PAHs) in refined olive and refined pomace olive oils using high performance liquid chromatography coupled with a fluorescence detector. Materials and Methods: The sample preparation involved liquid–liquid extraction with organic solvent and low temperature clean-up. The low temp...

A reversed-phase salt assisted liquid-liquid extraction (RP-SALLE) method was developed for the preconcentration and determination of trace crocin in herbal medicines by high-performance liquid chromatography (HPLC). In developed method, the roles of the aqueous and organic phases are inverted so that the analyte is extracted from the organic into the aqueous phase after salt addition and phase...

Solid phase extraction (SPE) and supercritical fluid extraction (SFE) have become established in the field of analytical chemistry over the last five years. Both techniques are alternatives to classical analytical extraction methods used to separate analytes from solid and liquid matrices. SFE is an alternative to traditional liquid-solid extraction methods, the two most common being soxhlet an...