Acridine orange enhances the uptake of [(3)H]actinomycin D in disrupted and intact human lymphocytes, as measured by liquid scintillation and autoradiography. Proflavine, quinacrine, chloroquine, and ethidium bromide are not effective. In mice, acridine orange increases the capacity of actinomycin to reduce spleen weight. Type II acridine binding to DNA may be a prerequisite for actinomycin enh...

Acetylcarnitine and acylcarnitine are hydrolyzed by alkaline hydrolysis. The total carnitine is measured by incubation of the sample with 14CacetylCoA and carnitine acetyltransferase. The radioactive acetylcarnitine is separated from the unreacted radioactive acetylCoA using a Dowex 2 x 8 anion exchange column. The radioactive acetylcarnitine fraction is quantitated by counting in a liquid scin...