this study was undertaken to estimate specific activities of rhodanese and 3-mercaptopyruvate sulfurtransferase (mst) in different tissues of turkey. enzyme activities were determined in tissue samples from freshly killed adult male turkeys by measurement of thiocyanate amounts produced by the action of enzymes on suitable substrates. activities of rhodanese and mst in examined tissues of turke...

Insulin resistance associated with visceral fat obesity has been suggested to be the pathological basis of metabolic syndrome. Many studies have demonstrated increased oxidant stress in diabetic patients and animal models of diabetes mellitus. In this study, the effect of liver hydrolysate administration on the blood glucose was examined in SHR/NDmcr-cp (SHR-cp) rats that show spontaneously occ...

Distribution of transglutaminase activity was determined in normal rat liver, a 3'-methyl-4-dimethylaminoazobenzene-induced primary hepatoma, and the Novikoff hepatoma. Over 90% of the total enzyme activity was found in the 105,000 X g supernatant of normal liver, whereas only 30% was found in this fraction of the hepatomas, the remainder being found in the particulate fraction. The is distribu...

It has been suggested that intracellular Hyal-1 (hyaluronidase-1), which is considered a lysosomal enzyme, originates via endocytosis of the serum enzyme. To test this proposal we have investigated the uptake and intracellular distribution of rhHyal-1 (recombinant human Hyal-1) by mouse liver, making use of centrifugation methods. Experiments were performed on wild-type mice injected with 125I-...

The 7- to 10-fold increase in the rat liver serine:pyruvate aminotransferase activity after glucagon administration was shown to occur mainly in the mitochondrial matrix of parenchymal cells. The enzyme was purified from glucagon-treated rat liver mitochondria to apparent homogeneity as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A specific rabbit antibody was prepared ...

The change in glutamine synthetase activity has been studied under two conditions of liver growth, namely, mitotic growth after partial hepatectomy and nonmitotic growth during repletion after a fast. The surgical operation caused the enzyme activity in the liver to diminish to one half its normal value within one week after the operation and to remain at this reduced level for the duration of ...