One of the most important virulence factors in Pseudomonas aeruginosa is biofilm formation, as it works a barrier for entering antibiotics into bacterial cell. Different environmental and nutritional conditions were used to optimize formation using microtitre plate assay by P. aeruginosa. The low nutrient level medium represented tryptic soy broth (TSB) was better than high with Luria Broth (LB...

Approximately 280 Escherichia coli isolates were isolated from a bovine feedlot at the University of Connecticut campus via enrichment in lauryl tryptose broth and random selection from MacConkey plates. The E. coli subspecies diversity was estimated by employing whole-cell BOX-PCR genomic fingerprints. A total of 89 distinct operational taxonomic units (OTUs) were identified by employing a cri...

Escherichia coli and Salmonella typhimurium strains grown in Luria-Bertani medium containing glucose secrete a small soluble heat labile organic molecule that is involved in intercellular communication. The factor is not produced when the strains are grown in Luria-Bertani medium in the absence of glucose. Maximal secretion of the substance occurs in midexponential phase, and the extracellular ...

In E. coli, the periplasmic proteins HdeA and HdeB have chaperone-like functions, suppressing aggregation of periplasmic proteins under acidic conditions. A microarray analysis of RNA isolated from an E. coli wild type and a ΔgcvB strain grown to mid-log phase in Luria-Bertani broth indicated the hdeAB operon, encoding the HdeA and HdeB proteins, is regulated by the sRNA GcvB. We wanted to veri...

Heavy metal pollution by natural factors is a world-wide phenomenon. Release of large quantities of heavy metals without handling proper processes that could decrease the concentration of such a material is a hassle that makes strains resistant to these heavy metals apart from entering into human food chain. In this research, wastewater of four firms in Guilan province such as Foolad, Risandegi...