Multiplex Ligation-Dependent Probe Amplification (MLPA) was used to study the integrity of chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in presence absence ionizing radiation. WTK1 cells contain a p53 mutation, whereas TK6 line has native tumor-suppressor gene. Each isolated pre- post-irradiation (2 3 Gy) analyzed by MLPA. Using probes that target specific regions o...

We have previously demonstrated that human red cell (RBC) phosphofructokinase (PFK) is under dual genetic control. since it is a mixture of five tetrameric isozymes composed of two distinct subunits. M (muscle-type) and I (liver-type). i.e.. M4. M31. M212. MI3. and 14. The residual RBC PFK in a patient with Tarui’s disease (glycogenosis type VII) was shown to consist exclusively of I type isozy...

The tyrosine kinase Tec belongs to a new group of structurally related nonreceptor tyrosine kinases that also includes Btk and Itk. Previous studies have suggested that these kinases have lineage-specific roles, with Tec being involved mainly in the regulation of cytokine-mediated myeloid cell growth and differentiation. In this study, we investigated expression and activation of Tec in human B...