We determined whether lyophilized High Five (H5) insect cells engineered to produce IFN-beta (H5BVIFN-beta) could induce systemic immunity against occult brain metastases. C3H/HeN mice were injected s.c. with syngeneic UV-2237M fibrosarcoma or K-1735M2 melanoma cells. Intralesional injection of 2 x 10(6) lyophilized H5BVIFN-beta cells produced complete regression of the s.c. tumors. Six weeks l...

Two experiments were conducted using 2 ruminally and duodenally fistulated steers to determine the digestibility of undegradable intake protein (UIP) of smooth bromegrass (Bromis inermis), birdsfoot trefoil (Lotus coniculatus L.), and heat-treated alfalfa (Medicago sativa) using the mobile nylon bag technique. Undegradable intake protein was determined using neutral detergent insoluble CP at a ...

Infrared-based (IR) thermal imaging data was combined here with mathematical modeling to describe the freezing process of a pharmaceutical formulation being lyophilized using two different loading configurations; (i) vials in direct contact shelf and (ii) suspended over it. In all experiments, nucleation event trigged at specific time instant vacuum induced surface (VISF) method. The IR given a...

Cefdinir (Cef) is a third-generation cephalosporin antibacterial agent with an extended spectrum and low solubility permeability. In this study, cefdinir was prepared as nanosuspension lyophilized to powder. The further processed develop bilayer tablet. By utilizing the approach, first therapeutic action impacted by immediate part's rapid disintegration prolonged release from floating part achi...

An artificial diet to mass-rear Euschistus heros (F. 1798) (Hemiptera: Pentatomidae) was developed in the laboratory. Biological studies were conducted under controlled conditions of temperature (25 ± 2 °C), RH: 60 ± 10%, and photoperiod of 14:10 (L:D) h. Out of 13 diets tested, 2 diets (D9 and D11) were the most suitable. The artificial diets selected had the same composition (green beans, pea...

This improved isotope-dilution gas chromatographic/mass spectrometric (GC/MS) method, in which [13C]glucose is the internal standard, meets the requirements of a Definitive Method. In a first study with five reconstituted lyophilized sera, a nested analysis of variance of GC/MS values indicated considerable among-vial variation. The CV for 32 measurements per serum ranged from 0.5 to 0.9%. Howe...

We have previously demonstrated that a low dose of live myeloma FO cells induced a cellular immunity against tumor without additional modulating factors. In the present study, lyophilized myeloma FO cells were used to induce anti-tumor immunity. In a myeloma vaccination model, immunization with lyophilized myeloma FO cells alone induced a slight response. However, the immunity was dramatically ...

radiolabeled microspheres of human serum albumin (msa) is of great importance in the analysis of regional lung perfusion. preparation of msa kit has been installed in our division recently is carried out in two stages:1. production of albumin sphere particles with suitable sizes by using the spinning disk technique.2. formulation of msa as a radiopharmacutical kit. so it can be labelled simphy ...

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background: this study aimed at determining the appropriate method for dewatering and drying biomass and selecting a suitable organic solvent for lipid extraction. methods: nannochloropsis oculata was cultured in gillard f/2 medium and after reaching the end of the stationary growth phase, algal biomass was separated from aqueous by centrifuge and dried through three methods: oven, air-dried an...