Treatment of Ehrlich ascites cells with 2 mM oleic acid causes a greater than 10-fold increase in the formation of platelet-activating factor (PAF; 1-[3H]alkyl-2-acetyl-sn-glycero-3-phosphocholine) from the de novo precursor of PAF, 1-[3H]alkyl-2-acetyl-sn-glycerol. Under these conditions, CTP:phosphocholine cytidylyltransferase activity, which is known to catalyze the rate-limiting step in pho...

During Alzheimer's Disease, sustained exposure to amyloid-β₄₂ oligomers perturbs metabolism of ether-linked glycerophospholipids defined by a saturated 16 carbon chain at the sn-1 position. The intraneuronal accumulation of 1-O-hexadecyl-2-acetyl-sn-glycerophosphocholine (C16:0 PAF), but not its immediate precursor 1-O-hexadecyl-sn-glycerophosphocholine (C16:0 lyso-PAF), participates in signali...

Abstract Background Fabry disease, an X-linked inborn error of metabolism, results from pathogenic mutations in the α-galactosidase A gene (GLA). These reduce or abolish activity, which result accumulation glycosphingolipids lysosomes, including globotriaosyl-sphingosinein (lyso-GB3), throughout body, particular kidney, heart, and brain. Lyso-GB3 may be elevated symptomatic patients supports a ...

  Combined PET/CT scanners now play a major role in medicine for in vivo imaging in oncology, cardiology, neurology, and psychiatry. As the performance of a scanner depends not only on the scintillating material but also on the scanner design, with regards to the advent of newer scanners, there is a need to optimize acquisition protocols as well as to compare scanner ...

Series of benzoolysis experiments with 1,2-dipalmitoyl glycerophosphocholine (GPC), 1-palmitoyl-2-lyso GPC, and 1-O-hexadecenyl-2-oleoyl GPC, as well as a few experiments with 1-oleoyl-2-acetyl GPC and 1-O-hexadecyl-2-acetyl GPC are reported. The results are used to present a consistent picture of the various dephosphorylation and benzoylation reactions occurring in molten benzoic anhydride.

The pathogenesis of gastroesophageal reflux disease (GERD) remains elusive, but recent evidence suggests that early secretion of inflammatory cytokines and chemokines by the mucosa leads to influx of immune cells followed by tissue damage. We previously showed that exposure of esophageal mucosa to HCl causes ATP release, resulting in activation of acetyl-CoA:1-O-alkyl-sn-glycero-3-phosphocholin...

A unique feature of lipoprotein lipase (LpL), the rate-limiting enzyme in the hydrolysis of circulating triglycerides, is its movement from its cell of synthesis, adipocyte or myocyte, to its site of action, the luminal endothelial surface. This involves processes that allow LpL to be released from the adipocyte cell surface and transferred against the flow of interstitial fluid to the luminal ...

We hypothesized that vitamin E (α-tocopherol) is required by the developing embryonic brain to prevent depletion of highly polyunsaturated fatty acids, especially docosahexaenoic acid (DHA, 22:6), the loss of which we predicted would underlie abnormal morphological and behavioral outcomes. Therefore, we fed adult 5D zebrafish (Danio rerio) defined diets without (E-) or with added α-tocopherol (...