The lysosome, initially discovered by Christian de Duve in 1955, is an intracellular organelle responsible of the ordered degradation of proteins, glycoproteins, proteoglycans, lipids, and other macromolecules originated from autophagy, endocytosis and phagocytosis. It is characterize by a limiting external membrane containing intraluminal vesicles. These organelles are estimated to contain 50-...

BACKGROUND Telavancin is a lipoglycopeptide with multiple mechanisms of action that include membrane-destabilizing effects towards bacterial cells. It shows bactericidal activity against forms of Staphylococcus aureus (phagolysosomal infection) with different resistance phenotypes [methicillin-resistant S. aureus, vancomycin-intermediate S. aureus or vancomycin-resistant S. aureus]. We examine ...

The structural and functional integrity of cytoplasmic organelles is maintained by intracellular mechanisms that sort and target newly synthesized proteins to their appropriate cellular locations. In melanocytic cells, melanin pigment is synthesized in specialized organelles, melanosomes. A family of melanocyte-specific proteins, known as tyrosinase-related proteins that regulate melanin pigmen...

the aim of the present study was to evaluate the antioxidant effects of aqueous fruit extract of ziziphusjujuba on ethanol-induced oxidative stress and to examine its protective effects on sperm motility and plasma membrane integrity of male wistar rats. adult male rats (n = 28) were divided into four equal groups: the first group served as untreated controls, the second group of rats was given...

the aim of this study was to determine the effect of low levels of glutathione on post-thawed buck sperm quality. in this experiment, different concentrations of glutathione [0 (lg-0), 0.5 (lg-0.5), 1 (lg-1), 1.5 (lg-1.5), and 2 (lg-2) mm] were added in a soybean lecithin-based extender. a total of 16 ejaculates from four bucks were collected and pooled. each pooled sample was divided into five...

Lysosomes are acidic compartments in mammalian cells that are primarily responsible for the breakdown of endocytic and autophagic substrates such as membranes, proteins, and lipids into their basic building blocks. Lysosomal storage diseases (LSDs) are a group of metabolic disorders caused by genetic mutations in lysosomal hydrolases required for catabolic degradation, mutations in lysosomal me...

Objective This study aimed to investigate the damage mechanism of nanosized hydroxyapatite (nano-HAp) on mouse aortic smooth muscle cells (MOVASs) and the injury-inhibiting effects of diethyl citrate (Et2Cit) and sodium citrate (Na3Cit) to develop new drugs that can simultaneously induce anticoagulation and inhibit vascular calcification. Methods The change in cell viability was evaluated usi...

The aim of the present study was to evaluate the antioxidant effects of aqueous fruit extract of Ziziphusjujuba on ethanol-induced oxidative stress and to examine its protective effects on sperm motility and plasma membrane integrity of male Wistar rats. Adult male rats (n = 28) were divided into four equal groups: the first group served as untreated controls, the second group of rats was given...

Until recently, a modest number of approx. 40 lysosomal membrane proteins had been identified and even fewer were characterized in their function. In a proteomic study, using lysosomal membranes from human placenta we identified several candidate lysosomal membrane proteins and proved the lysosomal localization of two of them. In the present study, we demonstrate the lysosomal localization of t...