The objective of this study was to develop a new antimicrobial film, in which lysozyme was covalently attached onto two different ethylene vinyl alcohol copolymers (EVOH 29 and EVOH 44). The EVOH surface was modified with UV irradiation treatment to generate carboxylic acid groups, and lysozyme was covalently attached to the functionalized polymer surface. Surface characterization of control an...

OBJECTIVE DA-9601, an extract of Artemisia asiatica containing eupatilin and jaceosidin as active compounds, has been prescribed to treat gastritis in Asia. In recent times, sustained-release, floating gastroretentive (GR) tablets of DA-9601 are available on the market. In the present study, the physical properties and in vitro drug release profile, in vivo gastric residence time, and gastropro...

The role of plasma lysozyme of normal healthy subjects (NHS, n = 12) and active pulmonary tuberculosis (ATB) patients (n = 15) on the innate immune mechanism was studied by the binding activity of lysozyme on live Mycobacterium tuberculosis. Plasma samples of NHS and ATB patients treated with live M. tuberculosis for 4 h and 24 h time points showed a significant decrease in the plasma lysozyme ...

The Ca(2+)/calmodulin-dependent protein kinase type IV/Gr (CaMKIV/Gr) is a key effector of neuronal Ca(2+) signaling; its function was analyzed by targeted gene disruption in mice. CaMKIV/Gr-deficient mice exhibited impaired neuronal cAMP-responsive element binding protein (CREB) phosphorylation and Ca(2+)/CREB-dependent gene expression. They were also deficient in two forms of synaptic plastic...

The objective of this study was to determine the effect of irradiation of pomegranate seed (PS) on chemical composition, digestibility and kinetic of gas production. Pomegranate seeds were exposed to gamma ray (GR) and electron beam (EB) at doses of 5, 10, 15 and 20 kGy. Three ruminally fistulated rams were used for obtaining ruminal fluid for in vitro digestibility and gas production measuremen...

To assess the role of lysozyme in pulmonary host defense in vivo, transgenic mice expressing rat lysozyme cDNA in distal respiratory epithelial cells were generated. Two transgenic mouse lines were established in which the level of lysozyme protein in bronchoalveolar (BAL) lavage fluid was increased 2- or 4-fold relative to that in WT mice. Lung structure and cellular composition of BAL were no...

BACKGROUND Lysozyme is a muramidase that acts on the peptideoglycan wall of Gram positive bacteria, causing cell death. It plays part in innate immunity and is present in blood, external fluid, as well in lysossomal granules of the phagocytes. Primary Immunodeficiencies are a diverse group of illnesses that, as a result of abnormalities of the immune system, increase susceptibility to infection...

some specific and non-specific immune variables of rainbow trout were assessed following vaccination of fish with formalin killed cells (fkc) and fkc containing extra cellular products (ecp) of s. iniae. rainbow trout weighing 80-100 g were vaccinated by intraperitoneal (i.p), dip and oral routes using fkc and fkc plus ecp with or without fround adjuvant (fa) at 16-17°c.antibody titration, lyso...

Biological functions of lysozyme, including its antimicrobial, antitumor, and immune-modulatory activities have been suggested to be largely determined by the lipid binding properties of this protein. To gain further insight into these interactions on a molecular level the association of lysozyme to liposomes composed of either 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine or its mixtures wi...